河北医学2017，Vol.23Issue(3)：363-366,4.DOI:10.3969/j.issn.1006-6233.2017.03.004
丙戊酸钠协同紫杉醇对肺癌A549细胞株血管新生相关因子表达的影响
The Effects of VPA Combined with PTX on the Expression of Angiogenesis Related Factors in A549 Lung Cancer Cell Line
摘要
Abstract
Objective:To investigate the effects of valproic acid ,one of histone deacetylase(HDAC) inhibitors, combinations with PTX, on the expression of vascular endothelial growth factor (VEGF) and Ang-2 of the lung cancer cell line A549 cell.Explore their significance of cell proliferation, apoptosis and metastasis on A549 cell.Methods:Set the A549 cell to four groups:control group,PTX group,VPA group and VPA combined with PTX group.Determine the mRNA and protein of VEGF and Ang-2 by quantitative PCR and Western Blot.Results:The PTX group,VPA group and VPA combined with PTX can downregulate the VEGF and Ang-2 in mRNA and protein level.The combination group of lowest level of these two genes and protein.The difference between the groups was statistically significant.Conclusion:HDAC inhibitor valproic acid may depend on its regulation of histone to inhibite cell proliferation , promote cell apoptosis of A549 cell and inhibit the angiogenesis by regulating the VEGF and Ang-2 expression.关键词
肺癌/组蛋白脱乙酰化酶抑制剂/丙戊酸钠/血管内皮细胞生长因子/Ang-2Key words
Lung cancer/Histone deacetylase inhibitor/VPA/VEGF/Ang-2引用本文复制引用
朱翠敏,高菲菲,梁云微,连相尧,胡潺潺,李青山..丙戊酸钠协同紫杉醇对肺癌A549细胞株血管新生相关因子表达的影响[J].河北医学,2017,23(3):363-366,4.基金项目
河北省医学科学研究重点课题计划项目,(编号:20120163) （编号:20120163）
河北省承德市科学技术研究与发展计划项目,(编号:201422027) （编号:201422027）
承德医学院附属医院科研青年基金项目,(编号:201403) （编号:201403）
