河南农业科学2017,Vol.46Issue(3):25-28,4.DOI:10.15933/j.cnki.1004-3268.2017.03.005
小麦T型细胞质雄性不育系T9023A的选育及育性恢复研究
Study on Breeding of T-type Wheat CMS Line T9023A and Its Fertility Restoration
摘要
Abstract
In order to enrich the resources of cytoplasmic male sterile(CMS) lines and restorer lines for T-type hybrid wheat,the elite wheat variety Zhengmai 9023 was used as the recurrent male parent to backcross with T-type CMS line T504A to breed new CMS line,and its male abortive characters as studied,the restorer lines were identified,the genetic analysis of fertility restoration were done.The results showed that a completely male sterile line T9023A was obtained through successive backcrossing.The anther of T9023A was shriveled,slender and no crack.The pollen grains of T9023A were irregular or spherical in shape and unstainable with I2-KI solution.By crossing T9023A with wheat variety Chuannong 26 and Chuannong 27(both having Avena fatua cytoplasm),two F1 hybrids were obtained.And their selfed seed fertility(domestic method) were 80.3% and 62.6%,respectively.The restoring capability of Chuannong 26 for T9023A was much higher.Segregation for fertility∶sterile in F2 populations of T9023A/Chuannong 26 fitted the ratio of 15∶1.This result suggested that Chuannong 26 contained 2 pairs of major restorer genes for T-type CMS.关键词
小麦/细胞质雄性不育/回交转育/育性恢复Key words
wheat/cytoplasmic male sterility(CMS)/backcross breeding/fertility restoration分类
农业科技引用本文复制引用
董普辉,朱先玉,程亚丹,望俊森,陶林伟,黄丁..小麦T型细胞质雄性不育系T9023A的选育及育性恢复研究[J].河南农业科学,2017,46(3):25-28,4.基金项目
国家自然科学基金项目(U1304320,U1304318) (U1304320,U1304318)
河南科技大学大学生科研训练计划项目(2015136) (2015136)