核农学报2017，Vol.31Issue(5)：884-888,5.DOI:10.11869/j.issn.100-8551.2017.05.0884
四环素类抗生素降解菌的分离与鉴定
Isolation and Identification of Tetracyclines Degrading Bacteria
摘要
Abstract
n order to identify potentially effective bacteria to degrade tetracyclines,6 strains were isolated from tetracycline-contaminated soils,and were further identified by the morphological,physiological and biochemical characteristics,and 16S rDNA sequence analysis.The degradation effects of this 6 strains were analyzed by high performance liquid chromatography.The results showed that all the six strains were able to grow in the medium supplemented with tetracycline as their sole carbon source.The degradation effects of strain TJ-2 towards oxytetracycline,tetracycline,and chlortetracycline were 58.33％,63.90％ and 65.50％,respectively.And also strain TJ-2 was higher effect compared to others strains in the study,which was followed by strain TJ6.After morphological,physiological and biochemical characteristics,and 16S rDNA sequence analysis on strain TJ-2,it was identified as Achromobacter xylosoxidans,while strain TJ-6 was identified as Bacillus subtilis.The results of our study provides practical basis for the microbial degradation of pesticide residues in soil.关键词
四环素类抗生素/降解菌/分离/鉴定/降解率Key words
tetracyclines/degrading bacteria/separation/identification/degradation rate引用本文复制引用
成洁,杜慧玲,张天宝,王炜..四环素类抗生素降解菌的分离与鉴定[J].核农学报,2017,31(5):884-888,5.基金项目
山西省高校“131”领军人才工程项目(2015053),山西农业大学科技创新基金(2011003) （2015053）
