核农学报2017，Vol.31Issue(6)：1145-1152,8.DOI:10.11869/j.issn.100-8551.2017.06.1145
辐照对瓜实蝇遗传区性品系成虫肠道微生物的影响
Effect of Irradiation on Bacterial Communities in the Gut of Adult Bactrocera cucurbitae From the Genetic Sexing Strain (Diptera: Tephritidae)
摘要
Abstract
In order to explore the influences of irradiation the diversity and structural composition of intestinal microflora in Bactrocera cucurbitae (Coquillett),adult females and males from B.cucurbitae genetic sexing strain (GSS) at different ages (5,8,11,13 d respectively) were irradiated by 137Cs at the dose of 100 Gy.Illumina MiSeq high-throughput sequencing platform was used to study the changes of gut microbial communities caused by irradiation.The results showed that 19 major bacteria genera were detected in adults'guts,of which 17 genera were known,and 1 was unclassified with 1 uncultured.The 17 known genera belong to 4 phyla,namely Proteobacteria (accounted for 50％ to 98％ of total amount),Firmicutes (accounted for 2％ to 47％ of total amount),Bacteroidetes (accounted for 1％ to 16％ of total amount) and one unclassified rare phyla (accounted for ＜ 1％ of total amount),respectively.In addition,the irradiation could significantly reduce the diversity and relative abundance of gut symbiotic bacteria in B.cucurbitae GSS adults,and the influences caused by irradiation had nothing to do with sex and adult ages.These results provide a reference for assessing and repairing the irradiation damage to B.cucurbitae.关键词
瓜实蝇遗传区性品系/成虫日龄/肠道微生物/辐照/Illumina MiseqKey words
Bactrocera cucurbitae GSS/adult age/gut microbial intestinal microflora/irradiation/Illumina MiSeq引用本文复制引用
姚明燕,张贺贺,向候君,季清娥,陈家骅..辐照对瓜实蝇遗传区性品系成虫肠道微生物的影响[J].核农学报,2017,31(6):1145-1152,8.基金项目
FAO/IAEA CRP(16035) （16035）
