核农学报2017，Vol.31Issue(6)：1153-1158,6.DOI:10.11869/j.issn.100-8551.2017.06.1153
快中子辐照对蝴蝶兰离体茎段生理特性的影响
Effect of Fast Neutron Irradiation on Physiological Characteristics of the Tissue-cultured Stem Segments of Phalaenopsis
摘要
Abstract
To explore the tolerance of phalaenopsis to fast neutron irradiation,the shoots of the tissue culture seedlings of Phalaenopsis Taisuco Firebird and Phalaenopsis Neyshanguniang were irradiated with fast neutron pulse reactor,and the effects of different effects of irradiation fluids on antioxidant enzyme activities,osmotic adjustment substances and membrane lipid peroxidation in plants were studied.The results showed that the activities of catalase (CAT),peroxidase (POD) and superoxide dismutase (SOD) in the leaf tissue of stem segments increased first and then decreased with the increase of irradiation trend.The lower dose (300 × l08 n· cm-2) irradiation promoted the activity of antioxidase and improved the anti-irradiation ability of Phalaenopsis.The higher dose (＞ 2 500 × 108 n· cm-2) irradiation inhibited the activity of antioxidase resulting in phloem injury of Phalaenopsis.Soluble protein content had significant negative correlation with fast neutron irradiation dose (r =-0.734,r =-0.669,p ＜ 0.01),while MDA content had significant positive correlation with fast neutron irradiation dose (r =0.929,r =0.926,p ＜ 0.01).In other words,low-dose irradiation promoted soluble protein anabolism and high-dose irradiation would exacerbate membrane lipid peroxidation.To sum up,Taisuco Firebird stems were more vulnerable to fast neutron irradiation than Neyshanguniang.The study provides a theoretical basis and technical reference for fast neutron irradiation on mutation breeding of flowers.关键词
快中子辐照/蝴蝶兰/生理特性Key words
fast neutron irradiation/phalaenopsis/physiological characteristics引用本文复制引用
李杰,张银洁,匡萍,喻兰,郑春..快中子辐照对蝴蝶兰离体茎段生理特性的影响[J].核农学报,2017,31(6):1153-1158,6.基金项目
四川省科技支撑计划(2016NZ0106),四川省科技应用基础项目(2017JY0132) （2016NZ0106）
