湖南农业大学学报（自然科学版）2017，Vol.43Issue(2)：135-139,5.DOI:10.13331/j.cnki.jhau.2017.02.004
仙人掌多糖对THP–1巨噬细胞源性泡沫细胞 胆固醇蓄积及其相关基因表达的影响
Effect of Opuntia dillenii Haw. polysaccharides on cholesterol accumulation and its associated gene expression in THP–1 macrophage–derived foam cells
摘要
Abstract
In this study, THP–1 cells were induced into macrophages and THP–1 macrophage–derived foam cell by phorbolmyristate acetate (PMA) and oxidized low–density lipoprotein (ox–LDL), and then treated with different concentrations(5, 10, 15 nmol/L) of ODP–Ⅰa, to investigate the beneficial effects of ODP–Ⅰa on the cholesterol accumulation in the THP–1 macrophage derived foam cell. Meanwhile, the mRNA expression and protein levels of ATP binding cassette transporter ABCA1, ATP binding cassette transporter ABCG1 and scavenger receptor class B type Ⅰ (SR–BI) that mediate cholesterol efflux were observed. The result showed that, the lipid content and the cholesterol content were increased significantly after the THP–1 cells were induced into the THP–1 macrophage derived foam cell, and then were significantly decreased after treating with 15 nmol/L ODP–Ⅰa. The data also demonstrated that, compared with the ox–LDL treated group, the high dose group (15 nmol/L ODP–Ⅰa treated group)showed significantly increase in the mRNA and protein expressions of ABCA1,ABCG1 and SR–BI(P<0.05). However, the high dose group was inferior to the ezetimibe treated group (P<0.05).And there were no significant differences among the low dose (5 nmol/L) group, the medium dose (10 nmol/L) group and the ox–LDL treated group. In summary, ODP–Ia promoted cholesterol efflux of THP–1 macrophage derived foam cell by up–regulation of ABCA1,ABCG1 and SR–BI.关键词
仙人掌多糖/THP–1巨噬细胞源性泡沫细胞/胆固醇蓄积Key words
Opuntia dillenii Haw. polysaccharides/THP–1 macrophage–derived foam cells/cholesterol accumulation分类
生物科学引用本文复制引用
李恒,罗齐军,卢向阳,曾富华..仙人掌多糖对THP–1巨噬细胞源性泡沫细胞 胆固醇蓄积及其相关基因表达的影响[J].湖南农业大学学报（自然科学版）,2017,43(2):135-139,5.基金项目
国家星火计划项目(2013GA780085) （2013GA780085）
广东省科技计划项目(2013B010404047) （2013B010404047）
广东省自然科学基金项目(2014A030307023,2016 A030313669) （2014A030307023,2016 A030313669）
湛江市科技攻关计划项目(2014A01012,2014A02012) （2014A01012,2014A02012）
