湖南农业大学学报（自然科学版）2017，Vol.43Issue(2)：187-194,8.DOI:10.13331/j.cnki.jhau.2017.02.014
鸭MyoG基因启动子的生物信息学分析及其 甲基化频率对基因表达的影响
Bioinformatics analysis on cloned promoter MyoG in duck, and the influence of its methylation levels on gene transcription
摘要
Abstract
Promoter sequence of gene MyoG in duck was amplified and cloned using RT–PCR, and its bioinformatic info was analyzed as well; the methylation level in CpG island (from –2536 to –1997 bp) of promoter MyoG in muscle tissues was detected using the Sequenom MassArray technique, meanwhile, its expression level was detected using qRT–PCR. The results showed that the amplified MyoG promoter sequences was 2730 bp which contained 2 CpG islands in promoter region. The CpG island (from –2536 to –1997 bp) included 5 binding sites of transcription factor and several eukaryotes structure components. The methylation data showed that methylation status from different individuals and tissues did not clustered together. Methylation frequency in CpG loci varied with individuals, and the methylation frequency on 22% of locus were negatively correlated withMyoG expression levels (P>0.05), whereas, on 78% of locus were positively correlated (P>0.05) with that, among of them, they reached significant difference level on locus of CpG 1, CpG 26.27.28.29 from leg muscles (P<0.05). The mechanism of regulating transcription for MyoG was different from mammals. Among the potential locus influencing MyoG transcription, CpG_1 and CpG_26.27.28.29 might affect MyoG transcription in skeletal muscle tissues of duck leg through DNA methylation. This study might list foundation data for studying the regulatory mechanisms of MyoG transcription in duck.关键词
鸭/肌细胞生成素/启动子/DNA甲基化Key words
duck/myogenin (MyoG)/promoter sequence/DNA methylation分类
农业科技引用本文复制引用
吴乾锋,刘贺贺,张涛,罗俊,胡博,王继文..鸭MyoG基因启动子的生物信息学分析及其 甲基化频率对基因表达的影响[J].湖南农业大学学报（自然科学版）,2017,43(2):187-194,8.基金项目
国家自然科学基金项目(31301964) （31301964）
四川省科学技术厅基金项目(2015JY0110) （2015JY0110）
