湖南农业大学学报（自然科学版）2017，Vol.43Issue(2)：195-199,5.DOI:10.13331/j.cnki.jhau.2017.02.015
湖南地区Ⅰ型乙型脑炎病毒的分离鉴定及E基因序列分析
Isolation and identification the Japanese encephalitis virus of genotype Ⅰ in Hunan province and sequence analysis on its E gene
摘要
Abstract
In order to explore Japanese encephalitis virus carried by mosquitoes in farms of Hunan Miluo, a JEV pathogenic investigation on mosquitoes was conducted in three farms of the region. Gene JEVNS5 collected from about 3900 mosquitoes in 3 pig farms was amplified using RT–PCR. The result showed that out of 17 in total 40 samples was positive. The JEV was isolated from the positive grinding fluid using the BHK–21 cell method. One JEV was obtained after 3 passages, and was named by HNML1. A pair of primer was designed according to gene JEVE, geneE from HNML1 was amplified, and sequencing and homology analysis were conducted as well. The results showed that the homology on nucleotide sequence and amino acid sequence of gene E to corresponding sequences in live antigen attenuated vaccine strains SA14–14–2 was 86.9% and 97.0%, respectively. There were 15 differences of amino acids, which included 8 key amino acids related to virulence. The result from gene homology analysis showed thatHNML1was a type strain of JEV Ⅰ.关键词
猪场/蚊子/基因I型/乙型脑炎病毒/湖南汨罗Key words
pig farm/mosquitoes/genotype Ⅰ/Janpanese encephalitis virus(JEV)/Hunan Miluo分类
农业科技引用本文复制引用
向卫军,杨涛涛,李润成,余兴龙..湖南地区Ⅰ型乙型脑炎病毒的分离鉴定及E基因序列分析[J].湖南农业大学学报（自然科学版）,2017,43(2):195-199,5.基金项目
湖南省科学技术厅重点课题(2012NK2001) （2012NK2001）
