江苏大学学报（医学版）2017，Vol.27Issue(2)：123-127,5.DOI:10.13312/j.issn.1671-7783.y170026
虫草素抑制脂多糖诱导的支气管上皮细胞炎症反应
Cordycepin suppresses lipopolysaccharide-induced inflammatory responsesin human bronchial epithelial cells
摘要
Abstract
Objective:To investigate the role of cordycepin in regulating the inflammatory response of human bronchial epithelial cells and its underlying mechanism.Methods:The human bronchial epithelial cells (16HBE),which grew in good condition,were treated with different concentrations of cordycepin (0,10,20,40,60 μmol/mL) for 4 hours and then treated with lipopolysaccharide(LPS,1 mg/L) for 12 hours.MTT assay was used for the detection of cell vitality.At the same time,the mRNA expression level of inflammatory cytokines (IL-8,TNF-α) was evaluated by quantitative real-time PCR.The level of p-AKT2 expression was analyzed by Western blotting analysis.Results:Cordycepin could protect bronchial epithelial cells from damage of LPS in a concentration-dependent manner(P<0.05),and attenuated LPS-induced release of pro-inflammatory cytokines(P<0.05),down-regulate the phosphorylation level of AKT2 induced by LPS(P<0.05).Conclusion:Cordycepin shows active inhibitory effect on the inflammatory response of 16HBE cells,possibly through mediating relevant signal pathway of AKT2.关键词
虫草素/支气管上皮细胞/炎症因子/AKT2Key words
cordycepin/human bronchial epithelial cells/inflammatory cytokines/AKT2分类
医药卫生引用本文复制引用
邓霞,郑丹丹,庄琼馨,柳星星,钱粉红..虫草素抑制脂多糖诱导的支气管上皮细胞炎症反应[J].江苏大学学报（医学版）,2017,27(2):123-127,5.基金项目
国家自然科学基金资助项目(81370119) （81370119）
镇江市社会发展项目(SH2015044) （SH2015044）
