解放军医学院学报2017，Vol.38Issue(4)：323-327,341,6.DOI:10.3969/j.issn.2095-5227.2017.04.009
膀胱癌相关基因的筛选及初步验证
Screening and verification for the relevant genes of bladder cancer
摘要
Abstract
Objective To screen bladder cancer related genes and analyze the correlation between the related genes and clinical features of patients with bladder cancer. Methods The gene expression in bladder cancer tissue and adjacent normal tissue were detected using gene chip assay. The genes with different expression in cancer tissue compared with adjacent tissue were selected and verified in 34 bladder cancer patients using q-PCR. The correlation of these genes with clinical features of bladder cancer patients were analyzed. Results Total of 6526 genes were found to be differently expressed in cancer tissue compared with adjacent tissue by gene chip assay. Twelve genes with greater differences were chosen for further identification in 34 samples. The expressions of four genes (CEACAM5, MYH11, CNN1 and LY6D) were found to be consistent with the results of gene chip assay. In the further investigation, we found that the levels of CEACAM5, MYH11 and CNN1 were significantly different between MIBC (muscle invasive bladder cancer) and NMIBC (non-muscle invasive bladder cancer). However, the level of LY6D in MIBC didn't differ with NMIBC. Meanwhile, the correlation of genes with other clinical features was not observed. Conclusion We reveals four genes related with bladder cancer in this study, and three of them (CEACAM5, MYH11 and CNN1) may involved in tumor invasion.关键词
基因表达谱芯片/膀胱癌/CEACAM5基因/MYH11基因/CNN1基因Key words
gene chip/bladder cancer/CEACAM5 gene/MYH11 gene/CNN1 gene分类
医药卫生引用本文复制引用
韩冬,刘成华,高亚萍,张旭,杨光,宋涛..膀胱癌相关基因的筛选及初步验证[J].解放军医学院学报,2017,38(4):323-327,341,6.基金项目
国家自然科学基金项目(81372745) Supported by the National Natural Science Foundation of China (81372745) （81372745）
