解放军医学院学报2017，Vol.38Issue(4)：337-341,5.DOI:10.3969/j.issn.2095-5227.2017.04.012
新型聚乙二醇水凝胶制备及性能分析
Development and characterisation of a noval polyethylene-glycol hydrogel
摘要
Abstract
Objective To synthesis a new polyethylene glycol hydrogel with rapid gelation, good mechanical properties, and hemostatic potential. Methods Noval hydrogels were prepared by physically blending the polymers (4-arm-PEG-NH2, 4-arm-PEG-NHS and 4-arm-PEG-CHO) with polyethylene glycol (PEG) in varying ratios (GEL-1: 1 : 1 : 0; GEL-2: 1 : 0.7 : 0.3;GEL-3: 1 : 0.5 : 0.5). The physicochemical properties were evaluated by vial tilting method, scanning electron microscopy (SEM), swelling tests and mechanical testing. In vitro cytotoxicity testing was done using the direct contact method by co-culture with mouse fibroblasts and quantitatively assessed by MTT assay. In vivo evaluation of hemostatic effect was carried out on rabbit liver parenchymal injury model with liver lobe excision. Results The gelling time of the new type of PEG hydrogel was short and adjustable. Scanning electron microscopy (SEM) showed that the novel hydrogel based on polyethylene glycol had a honeycomb-like porous structure with pores connected and evenly distributed, and the pore size could be controlled. The average swelling ratios of GEL-1, GEL-2 and GEL-3 were 1540%, 1620%, and 1780%, respectively. The mechanical property test showed that the compressive strength of the hydrogel decreased with the increase of the proportion of CHO, but the fracture stress was still 27MPa when the CHO accounted for 0.3. Co-culture with mouse fibroblasts in vitro showed that the new hydrogel had no obvious inhibitory effect on cell growth with low cytotoxicity. The new hydrogels demonstrated excellent hemostatic ability for 3-8 folds reduction in blood loss compared with the control. Conclusion The new hydrogel had a certain clinical application prospect as a hemostatic agent, and its application in the sustained-release drug delivery, tissue engineering and other fields need to be further explored.关键词
聚乙二醇/水凝胶/止血Key words
polyethylene glycol/hydrogel/hemostasis分类
医药卫生引用本文复制引用
全宸良,刘建恒,张里程,补亚忠,杨飞,高远,唐佩福..新型聚乙二醇水凝胶制备及性能分析[J].解放军医学院学报,2017,38(4):337-341,5.基金项目
部委级资助项目 （）
军队后勤课题面上项目(CWS14C066 （）
AWS14C003) Supported by the Foundation of Logistics of Chinese PLA (CWS14C066 （）
AWS14C003) （）
