解放军医学院学报2017，Vol.38Issue(4)：342-344,356,4.DOI:10.3969/j.issn.2095-5227.2017.04.013
免疫组织化学法在非小细胞肺癌EML4-ALK融合基因检测中的应用
Ventana IHC test for the echinoderm microtubule associated protein like 4-anaplastic lymphomakinase fusion gene in non-small cell lung cancer
范军振 1吕亚莉 1宋欣 1钟梅 1王琼 1朱凤伟 1孙海波 1石怀银1
作者信息
- 1. 解放军总医院 病理科,北京 100853
- 折叠
摘要
Abstract
Objective To explore the accuracy of Ventana IHC to detect EML4 - ALK in non-small cell lung cancer. Methods Total of 281 formalin-fixed paraffin-embedded samples (259 adenocarcinoma, 17 squamous carcinoma, 5 gland-scale cancer) diagnosed as NSCLC in Chinese PLA General Hospital from June 2014 to July 2016 were included in this study, and the EML4-ALK fusion gene of whom were detected by PCR and Ventana IHC, respectively. The PCR method was regarded as the golden standard, the positive rate between the two methods were compared and the sensitivity, specificity, positive predictive value and negative predictive value of Ventana IHC were calculated. Results The positive rates of PCR and Ventana IHC were 10.0% and 12.8%, respectively with no significant difference between the two methods (P=0.057). The sensitivity, specificity, positive predictive value and negative predictive value of Ventana IHC were 89.3%, 95.7%, 89.3% and 95.7%, respectively. Conclusion The Ventana IHC is efficient in detection of EML4 - ALK fusion gene of NSCLC with favorable sensitivity, specificity, positive predictive value and negative predictive value, and it is economical, simple and convenient to be performed.关键词
非小细胞肺癌/EML4-ALK融合基因/基因扩增/免疫组化Key words
non-small-cell lung cancer/EML4-ALK fusion gene/gene amplification/immunohistochemistry分类
医药卫生引用本文复制引用
范军振,吕亚莉,宋欣,钟梅,王琼,朱凤伟,孙海波,石怀银..免疫组织化学法在非小细胞肺癌EML4-ALK融合基因检测中的应用[J].解放军医学院学报,2017,38(4):342-344,356,4.
