临床口腔医学杂志2017，Vol.33Issue(4)：195-198,4.DOI:10.3969/j.issn.1003-1634.2017.04.002
不同患龋状态双胞胎儿童及其母亲牙菌斑中微生物多样性分析
HOMINGS analysis of plaque microflora in 15 mothers and 30 twins with discordant caries phenotypes
摘要
Abstract
Objective:The study was conductedto analyze and compare the oral microbial profiles of the twins with discordant phenotype and their mothers.Methods:Supragingival plaque samples are obtained from 14 paires of twins and a set of triplets with discordant caries phenotypes and 15 mothers,samples are assayed using the Human Oral Microbe Identification using Next Generation Sequencing(HOMINGS).Results:A total of 2,293,650 reads and 20431 OUT revealed 11 phyla,116 genus and 139 species of microflora,the relative abundance of these strains varied dramatically among the children.Comparative analysis between caries group and caries-free group revealed that the frequency of Actinomyces,Lactobacillus,Haemophilus and Streptococcus`s detection are much higher in caries group(P<0.05).The sample from the mother group revealed that the relative abundance of Prevotella,Tannerella were significant higher than caries group(P<0.05).Conclusion:The microbial diversity and abundance of mother group was significantly higher than that in caries group and caries-free group.Moreover,the difference of the oral microbiota between caries and caries-free group including Streptococcus,Actinomyces,Lactobacillus and Haemophilus are strongly associated with early childhood caries(ECC)and can be potential biomarkers for dental caries in primary dentition.关键词
双胞胎/龋病/龈上菌斑/母亲/微生物Key words
Twins/caries/Supragingival plaque/Mother/Microbial分类
医药卫生引用本文复制引用
郑玉巧,张萌,陈泳杏,江汉,李宇红,杜民权..不同患龋状态双胞胎儿童及其母亲牙菌斑中微生物多样性分析[J].临床口腔医学杂志,2017,33(4):195-198,4.基金项目
国家自然科学基金(81170954) （81170954）
