临床口腔医学杂志2017，Vol.33Issue(4)：211-213,3.DOI:10.3969/j.issn.1003-1634.2017.04.006
苦参碱对生物膜状态白色念珠菌ALS基因表达的影响
Influence of matrine on ALS gene expression in Candida Albicans biofilms
摘要
Abstract
Objective:To investigate the influence of different concentrations of matrine on the ALS gene expression in C.albicans biofilms.Methods:C.albicans collection strain ATCC76615 was obtained and biofilms were founded by slide method after following brief exposure to different concentrations of matrine in vitro.The levels of ALS1 and ALS4 mRNA expression were assessed by RT-PCR.Results:Matrine-treated cells expressed lower levels of ALS1 and ALS4 mRNA than the cells grown in the absence of matrine.High ALS mRNA was observed in normal mature biofilm.When the cells were treated with matrine,ALS decreased.The relative ALS1 was 0.96 and 0.34 at matrine concentrations of 1 mg/mL and 8 mg/mL,respectively.While the relative ALS4 was 0.93 and 0.28 at matrine concentrations of 1 mg/mL and 8 mg/mL,respectively.Conclusion:Matrine could inhibit C.albicans biofilms expressed lower levels of ALS1 and ALS4 mRNA in vitro.关键词
白色念珠菌/生物膜/黏附/ALS基因Key words
C.albicans/Biofilm/Adhesion/ALS gene分类
医药卫生引用本文复制引用
吴岚,沈雪敏,周曾同,葛姝云,周永梅..苦参碱对生物膜状态白色念珠菌ALS基因表达的影响[J].临床口腔医学杂志,2017,33(4):211-213,3.基金项目
国家自然科学基金资助项目(81400513)上海市科委资助项目(14401931600)2013-2014年度国家临床重点专科建设项目([2013]544) （81400513）
