临床口腔医学杂志2017，Vol.33Issue(4)：217-220,4.DOI:10.3969/j.issn.1003-1634.2017.04.008
干扰PTP4A1表达对人舌鳞癌细胞CAL27的影响
The effects of PTP4A1 gene interference on cell proliferation,apoptosis and migration ability in human tongue squamous cancer cells CAL27
黄宏伟 1马壮 1余志刚1
作者信息
- 1. 株洲市中心医院口腔科 湖南 株洲 412007
- 折叠
摘要
Abstract
Objective:To study the effects of PTP4A1 gene interference on cell proliferation,apoptosis and migration ability in human tongue squamous cancer cells CAL27 in vitro.Methods:Lentivirus transfection was used to realize the PTP4A1 gene interference in human tongue squamous cancer cells CAL27.PTP4A1 gene interference was verified by real-time PCR and western blotting.The cell proliferation was analysed by MTT method,and wound scratch assay was used to detect cell migration ability,the cell apoptosis rate was detected by flow cytometry.The apoptotic proteins expression levels of caspase-3,Bax and Bcl-2 were detected by western blotting.Results:The gene expression levels of PTP4A1 mRNA and protein in human tongue squamous cancer cells CAL27 were significantly decreased after interference lentivirus transfection.After the interference of PTP4A1,the cell proliferation and migratory ability of CAL27 cells in vitro were decreased(P<0.05).The cell apoptosis rate significantly increased after PTP4A1 gene interference(P<0.05),along with the caspase-3 and Bax protein expression levels up-regulation,and the Bcl-2 expression levels down-regulation.Conclusion:Gene interference of PTP4A1 in human tongue squamous cancer cells CAL27 inhibits the cell proliferation,migration ability in vitro,and enhances the cell apoptosis rate of CAL27 cells.关键词
人舌鳞癌细胞/PTP4A1基因/细胞增殖/凋亡/迁移Key words
Human tongue squamous cancer/PTP4A1 gene/Cell proliferation/Apoptosis/Migration分类
生物科学引用本文复制引用
黄宏伟,马壮,余志刚..干扰PTP4A1表达对人舌鳞癌细胞CAL27的影响[J].临床口腔医学杂志,2017,33(4):217-220,4.
