临床神经病学杂志2017，Vol.30Issue(2)：116-119,4.
雷帕霉素干预对左旋多巴诱发的异动症大鼠行为学的影响
Effect of Rapamycin treatment on the behavioristics of levodopa induced dyskinesia rats
摘要
Abstract
Objective To evaluate the effect of Rapamycin treatment on the behavioristics of levodopa induced dyskinesia (LID) rats.Methods Hemi-parkinsomism rat models were made by 6-hydraxydopamine microinjection stereotaxically into medial forebrain bundle.Then LID rat models were established by intraperitoneal injection of levodopa for a week.The LID rats were randomly divided into the LID control group (LID group) and Rapamycin treatment observation group (RAPA group).Then all of the rats were treated with levodopa by intraperitoneal injection for 2 weeks,and the rats in RAPA group were treated with Rapamycin by intraperitoneal injection 45 min before levodopa injection,with low dose of 0.25 mg/(kg·d),middle dose of 0.35 mg/(kg·d) and high dose of 0.5 mg/(kg·d),4 d a week for 2 weeks.The control group was given the same volume of normal saline.The abnormal involuntary movement (AIM) scores of rats were ecorded and analyzed.Results There was no significant behavior changes recorded in RAPA low dose subgroup.Rats in RAPA high dose subgroup appeared some problems such as listlessness,loss of appetite and hematuria after administration of Rapamycin for a few days,and died in about 5-10 d.The autopsy and pathologic diagnosis approved that the animals died due to pneumonia,liver and kidney function damage.The behavioral changes in RAPA middle dose subgroup were satisfying,and the animals showed well tolerance without rats died.After RAPA treatment,the dayly total AIM scores of the rats in RAPA middle dose subgroup were significantly lower than that in LID group (all P<0.05).The AIM scores of the RAPA middle dose subgroup within 120 min at 20 d after levodopa treatment were significantly lower than LID group (all P<0.05).Conclusion Rapamycin therapy can significantly improve the behavioral performance of LID rats,improve the involuntary movement symptoms of LID rats.关键词
帕金森病/左旋多巴诱发的异动症/雷帕霉素/行为学Key words
Parkinson's disease/levodopa induced dyskinesia/Rapamycin/behavioristics分类
医药卫生引用本文复制引用
张丙天,陈志斌,陈小武,缪茂军,何晓阔,曹学斌..雷帕霉素干预对左旋多巴诱发的异动症大鼠行为学的影响[J].临床神经病学杂志,2017,30(2):116-119,4.基金项目
国家自然科学基金(31260241) （31260241）
