临床误诊误治2017，Vol.30Issue(4)：99-103,5.DOI:10.3969/j.issn.1002-3429.2017.04.035
甘草酸二铵对百草枯诱导肺泡上皮Ⅱ型细胞高迁移率族蛋白1的影响
Effects of Diammonium Glycyrrhizinate on the Expression of HMGB1 in Alveolar Epithelial Cell with Paraquat
摘要
Abstract
Objective To investigate the effects of Diammonium Glycyrrhizinate (DG) on the expression of HMGB1 in rat alveolar epithelial cell Ⅱ(AECⅡ)with paraquat (PQ). Methods The AECⅡ was cultured in RPMI-1640 media containing 10% FBS, 100 U/ml penicillin and 100 μg/L streptomycin at 37°C in 5% CO2.The cells were divided into three groups: The NS group was induced 24 h without any drugs, the PQ group was induced 24 h by 1000 μmol/L PQ ,and the DG group was induced 2 h by 0.6 mg/ml DG, then, was induced 24 h by 0.6 mg/ml DG+1000 μmol/L PQ.MTT assay was used to measure the proliferation effect of DG on AEC Ⅱ with PQ.The ELISA assay was applied to measuring the levels of HMGB1, TLR-4,MyD88,NF-κB, TNF-α in the three groups.The gene expressions of HMGB1,TLR-4, MyD88, NF-κB P65 mRNA in the three groups were detected by R T-PCR.Results The highest survival rate of AECⅡ induced 24 h by 1000 μmol/L PQ was in the group of 0.6 mg/ml DG, the lowest survival rate of AECⅡ induced 24 h by 1000 μmol/L PQ was in the group of 0 mg/ml DG.The levels of HMGB1, TLR4, MyD88, NF-κB P65, TNF-α in PQ and DG group were higher than those in NS group.While, the levels of HMGB1, TLR-4, MyD88, NF-κB P65, TNF-α in DG group were lower than those in PQ group (P<0.01).The expression of HMGB1, TLR-4, MyD88, NF-κB P65, TNF-α mRNA in PQ and DG group were higher than those in NS group.The expression of HMGB1, TLR4,Myd88,NF-κB P65,TNF-αmRNA in DG group were lower than those in PQ group(P<0.01). Conclusion DG can attenuate the injury in rat alveolar epithelial cells caused by paraquat poisoning.And it can decrease the expressions of HMGB1, TLR-4, MyD88, NF-κB and TNF-α.关键词
甘草酸二铵/高迁移率族蛋白质类/百草枯Key words
Diammonium glycyrrhizinate/High mobility group proteins/Paraquat分类
医药卫生引用本文复制引用
方辰,张剑锋,喻莉,向沥..甘草酸二铵对百草枯诱导肺泡上皮Ⅱ型细胞高迁移率族蛋白1的影响[J].临床误诊误治,2017,30(4):99-103,5.基金项目
国家自然科学基金项目(81360290) （81360290）
广西自然科学基金项目(2014GXNSFAA118195) （2014GXNSFAA118195）
