国际眼科杂志2017，Vol.17Issue(5)：850-853,4.DOI:10.3980/j.issn.1672-5123.2017.5.11
CD105评价脉络膜新生血管变化的实验研究
An experimental research on changes of choroid neovascularization evaluated by CD105
摘要
Abstract
AIM:To observe the accuracy of the experimental choroid neovascularization(CNV) changes evaluated by CD105 immunohistochemical examination.METHODS:Twenty-four male Brown Norway(BN) rats were randomly divided into the control group (6 rats) and the experimental group (18 rats).The CNV were induced by 659nm krypton laser.And the power was 360mW,exposure time was 0.05s,spot diameter was 50μm.The formation rate of CNV and the average optical density(AOD) of leakage flare were observed by fluorescein fundus angiography(FFA) and indocyanine green angiography(ICGA) on the seventh day,the fourteenth day and the twenty-first day after laser photocoagulation.The histopathology changes of CNV and the AOD of CD105 were observed by eyeball exemplar.RESULTS:The CNV appeared on the seventh day after laser photocoagulation,and reached the peak on the fourteenth day and twenty-first day after laser photocoagulation.The formation rate of CNV were 77.08%,85.42%,89.58%,at 7,14 and 21d after laser photocoagulation.From 7 to 21d after laser photocoagulation,the AOD of leakage flare increased gradually(P<0.05).There had significant differences between 7 days` outcome and 14 days` outcome(P<0.05),and had no significant differences between 14 days` outcome and 21 days` outcome(P>0.05).From 7 to 21d after laser photocoagulation,the expression of CD105 increased gradually,and then decreased gradually,and from 14 to 21d,there had significant differences between 7 days` outcome and 14 days` outcome of AOD(P<0.05),and had no significant differences between 14 days` outcome and 21 days` outcome(P>0.05).CONCLUSION:Immunohistochemical outcomes of CD105 are highly consistent with fundus angiography outcomes of CNV changing regularity.关键词
脉络膜新生血管/CD105/氪激光Key words
choroidal neovascularization/CD105/krypton laser引用本文复制引用
陶方方,亢泽锋,刘健,褚文丽,周志豪..CD105评价脉络膜新生血管变化的实验研究[J].国际眼科杂志,2017,17(5):850-853,4.基金项目
National Natural Science Foundation of China (No.81574032) （No.81574032）
国家自然科学基金资助项目(No.81574032) （No.81574032）
