生态与农村环境学报2017，Vol.33Issue(3)：275-280,6.DOI:10.11934/j.issn.1673-4831.2017.03.012
一株高效凤眼莲脱胶菌的筛选及鉴定
Screening and Identification of an Efficient Strain of Bacteria Degumming Eichhornia crassipes
摘要
Abstract
Pretreatment is the most expensive and critical step of converting biomass into energy, while biological pretreatment has attracted extensive attention for its environmental friendliness and low energy consumption.In order to find a bacteria efficient for use in biological pretreatment, degumming efficiency was chosen as the scale for strain screening.A strain, coded as A1, was isolated from the environmental media where Eichhornia crassipes grew through enrichment culture and primary screening with the Congo red plate staining method, and tested to be a strain quite efficient in degumming water hyacinth.Under the optimal conditions, Strain A1 could secrete pectinase, xylanase and cellulase as high as 3 925.00, 8 331.67 and 4 883.62 nmol·s-1·mL-1, respectively, in activity.When used in pretreatment, it could reduce the weight of water hyacinth by 33.37% through one round of degumming, and cumulatively by 49.26% after three rounds of degumming.The strain was identified as Bacillus amyloliquefaciens by means of 16S rRNA sequencing and phylogenetic tree analysis.关键词
脱胶/凤眼莲/筛选/解淀粉芽孢杆菌Key words
degumming/Eichhornia crassipes/screening/Bacillus amyloliquefaciens分类
资源环境引用本文复制引用
杨庆,张超奇,马丽晓,周文兵,朱端卫,周易勇,肖乃东..一株高效凤眼莲脱胶菌的筛选及鉴定[J].生态与农村环境学报,2017,33(3):275-280,6.基金项目
中央高校基本科研业务费专项(2014PY061,2015BQ013,2016PY049) （2014PY061,2015BQ013,2016PY049）
武汉市关键技术攻关计划(2015020202010126) （2015020202010126）
教育部留学回国人员科研启动基金 （）
国家水体污染控制与治理科技重大专项(2012ZX07104-001) （2012ZX07104-001）
淡水生态与生物技术国家重点实验室开放课题(2016FB19) （2016FB19）
