中南医学科学杂志2017，Vol.45Issue(2)：117-121,5.DOI:10.15972/j.cnki.43-1509/r.2017.02.003
橘皮素对PC-12细胞Aβ水平的影响及机制研究
The influence and mechanism research of tangeretin onthe PC-12 cell beta amyloid protein levels
摘要
Abstract
Objective To observe the effect that the Tangeretin on the PC-12 cell beta Amyloid (Aβ) protein levels and discuss the possible mechanism.Methods Using different concentrations (0.1,1,10,100μmol/L) of the Tangeretin to treat PC-12 cells at different time (0h,6h,12h,24 h,48 h),the level of Aβ40and Aβ42inthe cell culture were detected by the method of ELISA,the levels of ATP binding cassette transporter A1 (ABCA1) protein in the PC-12 cells were detected by western blot,the levels of ABCA1 mRNA in the PC-12 cells were detected by RT-PCR.After using ABCA1 SiRNA to inhibit the expression of ABCA1 protein.Results Aβ40 and Aβ42 levels decreased significantly in 10μmol/L and 100μmol/L Tangeretin group,ABCA1 protein level increased significantly,and ABCA1 mRNA level of all groups didn`t change.With the treatment of 100μmol/L Tangeretin on PC-12 cells,ABCA1 mRNA expression hadn`t significant difference compared with the control group,ABCA1 protein level of 24 h and 48 h group increased significantly,Aβ40 and Aβ42 levels decreased significantly.Conclusion The Taneretin can reduce Aβ40and Aβ42 levels through increasing ABCA1 protein level.关键词
橘皮素/PC12细胞/ABCA1/β淀粉样蛋白/阿尔茨海默病Key words
tangeretin/PC-12 cells/ABCA1/amyloid beta/alzheimer`s disease分类
医药卫生引用本文复制引用
欧阳新平,郑莉,周寿红,陆素青,陈歆,张敏,何平平,唐朝克..橘皮素对PC-12细胞Aβ水平的影响及机制研究[J].中南医学科学杂志,2017,45(2):117-121,5.基金项目
湖南省研究生科研创新项目(CX2014B393) （CX2014B393）
湖南省中医药管理局重点项目(201524) （201524）
南华大学校内博士启动基金(2014XQD36、2015XQD37). （2014XQD36、2015XQD37）
