中南医学科学杂志2017，Vol.45Issue(2)：127-131,5.DOI:10.15972/j.cnki.43-1509/r.2017.02.005
丝裂霉素对胃癌细胞SGC-7901miRNA表达谱的影响
MicroRNA profiling of gastric cancer SGC-7901 aftermitomycin C treatment
摘要
Abstract
Objective To evaluate the mitomycin C (MMC) treatment on the miRNA expression profile in gastric cancer cells,and provide the theoretical evidences for MMC treatment on gastric cancer.Methods The effects and optimum concentration of MMC were evaluated in the cultured SGC-7901 cells by MMT.The miRNA microarray technique was used for the exploration of miRNA profile of SGC-7901 cells after MMC treatment and the different expression of candidate miRNA was further confirmed by qRT-PCR.Results The inhibition rate of SGC-7901 cells was negatively correlated with the MMC concentration.The 60 miRNAs have changed expression in SGC-7901 cells 48 hours after MMC treatment.Among them,11 miRNAs included miR-498 and miR-1247 were up-regulated,and miR-205* and miR-3924 were down-regulated.Some abnormal expression changes recovered after MMC treatment.Conclusion MMC can regulate the expression of 60 miRNAs including miR-498 in gastric cancer cells.关键词
丝裂霉素/MiRNA表达谱/胃癌/SGC-7901Key words
mitomycin/miRNA expression profile/gastric cancer/SGC-7901分类
医药卫生引用本文复制引用
谢娟,唐霞,李国庆,刘艳萍,李熠,冯聚玲,许丽芳,王超..丝裂霉素对胃癌细胞SGC-7901miRNA表达谱的影响[J].中南医学科学杂志,2017,45(2):127-131,5.基金项目
湖南省教育厅科学研究项目(16C1401)资助,湖南省教育厅优秀青年基金项目(13B098)资助. （16C1401）
