中南医学科学杂志2017，Vol.45Issue(2)：136-139,149,5.DOI:10.15972/j.cnki.43-1509/r.2017.02.007
乙酸致内脏痛模型小鼠杏仁中央核c-fos表达和动物焦虑样行为的影响
The expression of c-fos in the central nucleus of amygdala and its relationship with visceral pain related anxiety-like behaviour in mice
摘要
Abstract
Objective To explore the expression of c-fos in the central nucleus of amygdala (CeA) and its relationship with acetic acid-elicited visceral pain (AAVP)related anxiety behavior in mice.Methods Male Kunming mice were randomly divided into normal saline (NS)group and one hour after acetic acid-elicited visceral pain (AA-1h)group,two hours after acetic acid-elicited visceral pain (AA-2 h)group and twenty-four hours after acetic acid-elicited visceral pain (AA-24 h)group,visceral pain behaviours of all animals were measured by Writhing test while the anxiety-like behavior of NS group and AA-2 h group and AA-24 h group animals were determined by open field test (OFT).After the behavior test,the three group animals were scarified for the determination of c-fos in the CeA by immunohistochemistry.Results Compared to NS group, AA-1h group animals showed significantly increased number of writhes(P＜0.001),while no statistics difference between NS group and AA-2 h or AA-24 h group(P＞0.05,respectively).In the OFT,AA-2 h or AA-24 h group showed significantly decreased time and number of crossing in the center compared with the NS group(P＜0.001,respectively),while no difference in the locomotive activity was found among the three groups(P＞0.05,respectively).The results of immunohistochemistry showed that compared to NS group,AA-2 h or AA-24 h group showed significantly increased number of c-fos positive cell in the CeA(NS vs AA-2 h,P＜0.001,NS vs AA-24 h,P＜0.05).Conclusion AA treatment leads to anxiety-like behavior in the mice even after the visceral pain disappeared,which might be due to the over-activated neuron in the CeA.关键词
内脏痛/杏仁中央核/c-fos/焦虑Key words
visceral pain/central nucleus of amygdala/c-fos/anxiety分类
医药卫生引用本文复制引用
李林,曹文宇,刘政海,曾佳玉,徐杨,张仁丽,万炜,向宇燕..乙酸致内脏痛模型小鼠杏仁中央核c-fos表达和动物焦虑样行为的影响[J].中南医学科学杂志,2017,45(2):136-139,149,5.基金项目
湖南省教育厅重点项目(编号:12A119) ,南华大学博士科研启动基金(编号:2015XQD28),湖南省(南华大学)研究生科研创新项目(编号:CX2016B479),衡阳市科技发展计划项目(编号:2015KJ19). （编号:12A119）
