南京工业大学学报（自然科学版）2017，Vol.39Issue(2)：113-120,8.DOI:10.3969/j.issn.1671-7627.2017.02.019
金针菇中总多酚含量测定方法的优选
Optimization on determination of total polyphenol in Flammulina velutipes
摘要
Abstract
To optimize the conditions to determinate the total polyphenol in Flammulina velutipeswith validation results and other evaluating indicators,the applicability of Folin-Ciocalteu method,UV spectrophotometry and Prussian blue method was investigated,and the method for determination of total polyphenol in Flammulina velutipes was selected.With the absorbance as indicator,single-factor experiments of chromogenic conditions were studied,and the chromogenic conditions were optimized by quadratic orthogonal rotation combination design.Results showed that comparing the interference,sensitivity and stability of the three methods,Folin-Ciocalteu method was the optimal one for the determination of total polyphenol in Flammulina velutipes.The optimum chromogenic conditions were 2mL Folin-Ciocalteu reagent,2.4 mL 15%Na2CO3 at 45℃ for 70min.The method was accurate,stable and reliable for the determination of total polyphenol in Flammulina velutipes with greatly improved detection sensitivity.关键词
金针菇/多酚/含量测定/二次正交旋转组合设计Key words
Flammulina velutipes/polyphenol/content determination/quadratic orthogonal rotation combination design分类
医药卫生引用本文复制引用
汪佳丹,徐婷,韩伟..金针菇中总多酚含量测定方法的优选[J].南京工业大学学报（自然科学版）,2017,39(2):113-120,8.基金项目
上海市科技兴农重点攻关项目[沪农科攻字(2012)第2-9号] （2012）
