南阳师范学院学报2017，Vol.16Issue(3)：22-28,7.
基于高通量测序的鸭河口水库微型
High-throughput sequencing for the study of nanoplankton communitycomposition in Yahekou Reservoir
摘要
Abstract
This study is aimed at investigating the nanoplankton community composition in Yahekou Reservoir using high-throughput sequencing and classical light microscopy.Based on the rarefaction curves of high-throughput sequencing, the number of OTUs, principal coordinate analysis (PCA), and UPGMA, the samples obtained from the center of the reservoir had higher diversity and species evenness.Phylogenetic analysis showed that the nanoplankton community composition could be divided into 38 and 33 major phylogenetic groups by phyla, the predominant phytoplankton groups included Cryptophyta, Chlorophyta, Chrysophyta, and Bacillariophyta while the zooplankton groups included Metazoa, Dinoflagellata, Cercozoa, Ciliophora, Centrohelida, Stramenopiles, Amoebozoa, and Euglenozoa.In this study, nanoplankton composition was characterized by high-throughput sequencing and classical light microscopy, and the results of these analyses were compared.High-throughput sequencing detected more species than light microscopy did, and thus gave a better assessment of nanoplankton diversity.However, high-throughput sequencing of 18S rDNA sequences amplicons did not adequately identify nanoplankton at the species level, while light microscopy did.In summary, a combined strategy using both morphological and molecular techniques is recommended.关键词
鸭河口水库/微型浮游生物/浮游植物/浮游动物/群落组成/高通量测序Key words
Yahekou reservoir/nanoplankton/phytoplankton/zooplankton/community composition/high-throughput sequencing分类
生物科学引用本文复制引用
黄进,赵海军,朱静亚,鲁开杰,张亦晨,尹延震,李冰,尹行..基于高通量测序的鸭河口水库微型[J].南阳师范学院学报,2017,16(3):22-28,7.基金项目
国家"十二五"科技支撑计划(2012BAC06B03) （2012BAC06B03）
河南省科技厅项目(142102310210) （142102310210）
河南省教育厅项目(14A180015) （14A180015）
南阳师范学院高层次人才科研资助项目(ZX2014069) （ZX2014069）
