中国海洋大学学报（自然科学版）2017，Vol.47Issue(4)：52-57,6.DOI:10.16441/j.cnki.hdxb.20160185
基于高通量测序的青石斑鱼基因组微卫星开发及评价
Development and Population Genetic Diversity Analysis of Microsatellite Markers in Epinephelus awoara
摘要
Abstract
In this study,The microsatellites of Epinephelus awoara were isolated,and the genetic diversity were analysed.Firstly,High throughput sequencing technology was used to develop microsatellites of Epinephelus awoara,and 2.860 Gbp raw data was obtained.Microsatellites were identified by screening the genome sequences using software MISA(MIcroSAtellite),and 967 657 different kinds of repeat motif types of microsatellite sequences were discovered.Ninety-six pairs of primers were designed according to unique microsatellite flanking sequences for amplification by polymerase chain reaction (PCR) with the software Primer 5.0,among which 60 loci were clearly amplified and shown polymorphic.In this paper,16 polymorphic microsatellite loci were characterized to evaluate the genetic diversity of parent population (48 individuals included) of Epinephelus awoara.A total of 129 alleles were detected,the number of alleles per loci ranged from 4 to 14 and effective number of alleles ranged from 1.905 9 to 8.295 3 with the average of 7.166 7 and 4.241 1,respectively.The observed heterozygosities (Ho) were from 0.422 to 1.000 (mean=0.678 6),and the expected heterozygosities (HE) ranged from 0.481 to 0.893 (mean=0.676 8).Five loci deviated significantly from the Hardy-Weinberg equilibrium.Three markers indicated significant heterozygote deficiency including EAW-55596,EAW-21755 and EAW-30334.Otherwise,EAW-33674 and EAW-52520 indicated significant heterozygote excess.And none of the loci combinations showed significant linkage disequilibrium.The polymorphism information content (PIC) was from 0.362 3 to 0.872 2 (mean=0.639 7＞0.5),and this result indicated that the genetic diversity of population was at high level.Thus,these microsatellite loci first presented useful molecular markers in the analysis of the genetic variability and in the investigation of genetic relations of Epinephelus awoara,which will provide a basis for genetic breeding.关键词
青石斑鱼/高通量测序/微卫星标记/多态性/群体遗传评价Key words
Epinephelus awoara/high-throughput sequencing/microsatellite/polymorphism/genetic diversity of population分类
农业科技引用本文复制引用
高峰涛,邵长伟,崔忠凯,王升鹏,魏敏,陈松林,杨官品..基于高通量测序的青石斑鱼基因组微卫星开发及评价[J].中国海洋大学学报（自然科学版）,2017,47(4):52-57,6.基金项目
山东省泰山学者项目资助 （）
Supported by the Taishan Scholar Project of Shandong Province （）
