四川中医2017，Vol.35Issue(3)：40-43,4.
补肾中药单体对氧化损伤内皮细胞中p66mRNA的调控作用
Regulating Effect of Tonifying Kidney Monomer on p66 mRNA of Oxidative Endothelial Cells
摘要
Abstract
Objective:To study the regulation of p66 mRNA of six kinds of tonifying kidney monomer:geniposidic acid,oleanolic acid,psoralen,sesamin,specnuezhenide,cinnamic acid on oxidative endothelial cells and to explore the mechanisms of its anti-oxidation and protection of vascular endothelial cells.Methods:EA.hy926 (human umbilical vein endothelial cells) were induced by H2O2 to be the oxidative stress damage model of vascular endothelial cells.After the intervention of different concentrations of geniposidic acid,oleanolic acid,psoralen,sesamin,specnuezhenide,cinnamic acid,p66 mRNA in the oxidative endothelial cells was detected by qPCR assay,Results:The superoxide dismutase (SOD) activity was decreased.The content of malondialdehyde (MDA) and reactive oxygen species was increased after the stimulation of H2O2.5 μM,50μM,500μM of geniposidic acid,psoralen,sesamin,cinnamic,especially acid specnuezhenide and oleanolic acid,inhibited the level of p66 mRNA expression level of oxidative vascular endothelial cells model.Conclusions:The effect of anti-oxidation and protection of vascular endothelial cells of tonifying kidney monomer is related with the regulation of p66 mRNA expression level.关键词
补肾中药单体/EA.hy926/氧化应激/p66ShcKey words
Tonifying kidney monomer/EA.hy926/Oxidative stress/p66Shc分类
医药卫生引用本文复制引用
孙申维,顾闻,陈川,刘特,陈久林,申定珠,邢三丽,郁志华..补肾中药单体对氧化损伤内皮细胞中p66mRNA的调控作用[J].四川中医,2017,35(3):40-43,4.基金项目
国家自然科学基金资助项目(编号:81373706) （编号:81373706）
