山东科学2017，Vol.30Issue(2)：13-19,7.DOI:10.3976/j.issn.1002-4026.2017.02.003
基于特征图谱的黄芩黄酮类成分固相萃取研究
Solid phase extraction of flavonoids from Radix Scutellariae based on specific chromatogram
摘要
Abstract
The specific chromatogram of flavonoids in Radix Scutellariae was established by HPLC in this paper.The column filling,eluent and the elution condition of solid phase extraction were optimized using the recovery rates and specific spectral similarity of six main characteristic peaks in HPLC specific chromatogram as indexes.Experimental results confirmed that C18 solid phase extraction (SPE) possessed the highest recovery rates of six components.The SPE column was rinsed with methanol-0.3％ formic acid(20∶80,V/V),and then was eluted finally with methanol (containing 0.3％ formic acid).After being purified,the recovery rates of six components were all over 89％,and the similarity of characteristic chromatogram was higher than 0.98.Based on HPLC characteristic chromatogram,the optimized purification process of flavonoids in Radix Scutellariae with solid phase extraction can ensure that the main effective components remain consistent before and after purification of Radix Scutellariae.关键词
黄芩/HPLC特征图谱/固相萃取/黄酮类成分Key words
Radix Scutellariae/HPLC specific chromatogram/solid phase extraction/flavonoids分类
医药卫生引用本文复制引用
郭威,王亮,周倩,聂颖兰,崔宁,于宗渊..基于特征图谱的黄芩黄酮类成分固相萃取研究[J].山东科学,2017,30(2):13-19,7.基金项目
山东省中医药科技发展计划(2015-164) （2015-164）
