山东医药2017，Vol.57Issue(13)：9-12,4.DOI:10.3969/j.issn.1002-266X.2017.13.003
TRAIL逆转人胃腺癌SGC7901/ADR细胞多药耐药的机制探讨
Mechanism of TRAIL reversing multidrug resistance of human gastric adenocarcinoma SGC7901/ADR cells
摘要
Abstract
To investigate the influence of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on multidrug resistance gene (MDR1), multidrug resistance-associated protein (MRP1), anti-apoptotic gene Bcl-2 and pro-apoptotic gene Bax in human gastric cancer SGC7901/ADR cells and to explore the possible mechanism of TRAIL reversing multidrug resistance of gastric cancer.Methods The drug sensitivity of SGC7901/ADR cells and their parent cells SGC7901 to doxorubicin, vincristine and cisplatin was detected by MTT assay.The mRNA expression of MDR1, MRP1, Bcl-2, Bax gene of SGC7901/ADR and SGC7901 cells was detected by real-time fluorescence quantification PCR.SGC7901/ADR cells were treated with different concentrations of TRAIL (10, 50, 100 ng/mL).The real-time fluorescence quantification PCR and Western blotting were used to measure the expression of MDR1, MRP1, Bcl-2, Bax mRNA and protein of various genes in different treatment groups and the blank control group.Results MTT assay for drug sensitivity test showed that, compared with SGC7901 cells, the IC50 of SGC7901/ADR cells to doxorubicin, vincristine and sisplatin was significantly increased (P<0.01 or P<0.05).Compared with SGC7901, the expression levels of MDR1, MRP1, Bcl-2 mRNA in SGC7901/ADR cells were significantly increased, but the expression of Bax mRNA was decreased (all P<0.01).The mRNA and protein expression levels of MDR1, MRP1 and Bcl-2 in SGC7901/ADR cells were down-regulated by TRAIL, and the expression of pro-apoptotic protein Bax mRNA was up-regulated.Compared with the blank control group, there were statistically significant differences (all P<0.05).Conclusion TRAIL may down-regulate the expression of MDR1, MRP1, Bcl-2 and up-regulate the expression of Bax to promote the apoptosis of gastric cancer drug-resistant cells, and reverse multidrug resistance of gastric cancer drug-resistant cells.关键词
胃肿瘤/肿瘤坏死因子相关凋亡诱导配体/多药耐药/细胞凋亡Key words
stomach neoplasms/tumor necrosis factor-related apoptosis-inducing ligand/multidrug resistance/apoptosis分类
医药卫生引用本文复制引用
孙海兵,张开光,李琴,刘应玲,陈思..TRAIL逆转人胃腺癌SGC7901/ADR细胞多药耐药的机制探讨[J].山东医药,2017,57(13):9-12,4.基金项目
安徽省自然科学基金资助项目(1308085MH167). （1308085MH167）
