山东医药2017，Vol.57Issue(13)：28-31,4.DOI:10.3969/j.issn.1002-266X.2017.13.008
石榴皮总黄酮对高糖环境下人脐静脉内皮细胞损伤的保护作用及机制
Protective effect of total flavonoids of Pomegranate husk on injuries of human umbilical vein endothelial cells under high glucose
摘要
Abstract
To investigate the protective effect of total flavonoids of Pomegranate husk on injuries of human umbilical vein endothelial cells under high glucose.Methods The injuries of human umbilical vein endothelial cells (HUVECs) were induced by 0, 5, 10, 20, 40, 80 and 160 mmol/L glucose.MTT was used to detect the cell viability.The cells in the logarithmic phase were divided into the blank group, model group, positive control group, high concentration group, medium concentration group and low concentration group.In addition to the blank group, cells in the rest of the groups were added with a certain amount of glucose to the final concentration of 40 mmol/L, followed by the positive control group with 1×10-5 mmol/L aminoguanidine 50 μL, high, medium and low concentration groups with 1×10-4, 1×10-5 and 1×10-6 mmol/L total flavonoids of Pomegranate husk (50 μL in each group), and they were all cultured for 48 h.We observed the morphology of human umbilical vein endothelial cells.MTT assay was used to detect cell viability.The cell clyce, the levels of lactate dehydrogenase (LDH), intercellular adhesion molecule-1 (ICAM-1), NO and superoxide dismutase (SOD) were measured by the propidium iodide method.Results The optical density (OD) of HUVECs treated with glucose (≥40 mmol/L) was decreased as compared with that of HUVECs without adding glucose (P<0.05 or P<0.01).Compared with the blank group, in addition to high concentration group, the OD in the other groups was decreased (P<0.05 or P<0.01).Compared with the model group, the OD of the other groups was increased (P<0.05 or P<0.01).Forty mmol/L glucose caused cell cycle arrest in G0-G1 phase, and with the increase of flavonoids from Pomegranate husk, it effectively promoted the cell transformation from G0-G1 to S phase, but it had little effect on cells in the G2-M phase.Compared with the blank group, the levels of LDH and ICAM-1 were higher in the supernatant of the other groups except in the high concentration group, and the levels of SOD and NO were decreased.Compared with the model group, the levels of LDH and ICAM-1 in the supernatant of the positive control group, the high concentration group and the medium concentration group were decreased, the levels of SOD and NO were increased (P<0.05 or P<0.01), and the LDH level in the low concentration group was decreased (P<0.01).Conclusion Flavonoids from Pomegranate husk can significantly reduce the injuries of HUVECs under high glucose, promote the cell from G0-G1 to S phase, and promote cell proliferation, which may be related with the reduction of expression of adhesion molecules, and the increase of SOD and NO.关键词
人脐静脉内皮细胞/细胞损伤/石榴皮黄酮/高糖环境Key words
human umbilical vein endothelial/cell injuries/flavonoids from Pomegranate husk/under high glucose分类
医药卫生引用本文复制引用
张蕾,张必嘏,郭丽萍..石榴皮总黄酮对高糖环境下人脐静脉内皮细胞损伤的保护作用及机制[J].山东医药,2017,57(13):28-31,4.基金项目
上海市卫生和计划生育委员会科研课题(20144Y0235). （20144Y0235）
