山东医药2017，Vol.57Issue(14)：1-5,5.DOI:10.3969/j.issn.1002-266X.2017.14.001
1,25(OH)2D3对人肾小球系膜细胞凋亡活性的影响及机制探讨
Effect of 1,25(OH)2D3 on apoptosis of human mesangial cells and its mechanism
摘要
Abstract
Objective To investigate the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] on apoptosis and expression of apoptosis-related factors in human mesangial cells (HMC).Methods The cultured HMC were randomly divided into four groups: normal control group (group N,cultured in DMEM medium with 5% fetal bovine serum),epidermal growth factor (EGF) group (group E,cultured in DMEM medium plus 10 ng/mL EGF),1,25(OH)2D3 group (group V,cultured in DMEM medium plus 10-8 mol/L 1,25(OH)2D3),and the EGF+1,25(OH)2D3 group (group VE,cultured in DMEM medium plus 10 ng/mL EGF and 10-8 mol/L 1,25(OH)2D3),they were all treated for 48 h.The Caspase-3 activity was detected by the Casapase-3 kits.The protein expression of Akt,phosphorylated Aakt (p-Akt) Caspase-3,Caspase-8,and Caspase-9 was detected by Western blotting.Meanwhile,the mRNA expression of Caspase-3,Caspase-8,and Caspase-9 mRNA was detected by RT-PCR.Results The activity of Caspase-3 in the group E was lower than that in the group N,the activity of Caspase-3 in the groups V and EV was higher than that in the group N,and the activity of Caspase-3 in the group EV was higher than that in the group E (all P<0.05).The ratio of p-Akt/Akt in the group E was higher than that in the group N,and the ratio of p-Akt/Akt in the group V was lower than that in the group N,and the ratio of p-Akt/Akt in the group EV was lower than that in the group E (all P<0.05).The protein and mRNA expression of Caspase-3 and Caspase-9 in the group E was lower than that in the group N,the expression of Caspase-3 and Caspase-9 in the group V was higher than that in the group N,and the expression of Caspase-3 and Caspase-9 in the group EV was higher than that in the group E (all P<0.05).Conclusion 1,25(OH)2D3 may enhance the apoptosis activity of HMC and thus promote the apoptosis of HMC by up-regulating the expression of Caspase-3/9 and down-regulating the expression of p-Akt.关键词
人肾小球系膜细胞/1,25-羟维生素D3/表皮生长因子/细胞凋亡/蛋白激酶B/半胱氨酸蛋白酶Key words
human mesangial cells/1/25-Hydroxyvitamin D3/epidermal growth factor/apoptosis/protein kinase B/cysteine protease分类
医药卫生引用本文复制引用
顾杰,张昊,张春江,赵丹,刘刚,杨晓萍..1,25(OH)2D3对人肾小球系膜细胞凋亡活性的影响及机制探讨[J].山东医药,2017,57(14):1-5,5.基金项目
国家自然科学基金资助项目(81160090) （81160090）
新疆石河子大学高层次人才科研启动资金专项(RCZX201026). （RCZX201026）
