山东医药2017，Vol.57Issue(14)：14-17,4.DOI:10.3969/j.issn.1002-266X.2017.14.004
乌梅提取物对纳米细菌致大鼠肾结石形成的影响及机制探讨
Effect and mechanism of Fructus mume extracts on formation of kidney calculi of rats induced by nanobacteria
摘要
Abstract
Objective To investigate the effect and mechanism of Fructus mume extracts on the formation of the renal calcium oxalate stones in rat models induced by nanobacteria (NB).Methods Thirty SD rats were randomly divided into A,B,C,D,E five groups of 6 rats in each.Rats in the group A were treated with 1 mL normal saline by tail vein injection on the first day which was taken as the control group,rats in the other groups were treated with 1 mL suspension of NB by tail vein injection to establish the models of nephrolithiasis.From the second day,rats in the groups A and B were provided with 2 mL normal saline by gavage every day,and the rest of groups were provided with 2 mL Fructus mume extracts (0.05,0.10 and 0.15 g/mL) every day.After 4 weeks,we collected 24 h urine to measure the urine oxalate (Ox) level by potassium permanganate fading method,used automatic biochemical analyzer to determine the level of urine Ca2+,citrate kit to determine urinary citrate levels,8-OHDG test box to detect the urine 8-OHDG levels.The left kidney was examined and graded for crystal deposition using HE staining.The content of malondialdehyde (MDA) in renal tissues was determined using thiobarbituric acid method.Results The levels of Ox,Ca2+ and 8-OHDG in urine of the group B were higher than those in the group A,and the level of citric acid was lower than that of group A (all P<0.05).The levels of Ox,Ca2+ and 8-OHDG in urine of the groups C,D and E were lower than those in the group B,the level of citric acid was higher than that of group B (all P<0.05).No calcium-oxalate crystals were found in the group A,but the calcium-oxalate crystals were found in the group B with a large quantity,and the number of calcium-oxalate crystals in the groups C,D and E was significantly less than that of the group B.The scores of calcium-oxalate crystals in the group B were higher than those in the group A,and the scores of groups C,D and E were lower than those of group B (all P<0.05).The level of MDA in the renal tissues of group B was higher than that of group A,and the level of MDA in renal tissues of groups D and E was lower than that in the group B (all P<0.05).Conclusions Fructus mume extracts have significant inhibitory effect on renal calcium oxalate stones in rats induced by NB,and its mechanism may be related to the decrease of urine urinary Ox level,thereby reducing urinary excretion of Ca2+ and reducing lipid peroxidation damage.关键词
乌梅提取物/纳米细菌/肾结石/草酸/8-羟基脱氧鸟苷Key words
Fructus mume extracts/nanobacteria/kidney calculi/oxalic acid/8-hydroxy-2′-deoxyguanosine分类
医药卫生引用本文复制引用
刘淼,谷江,张永春,夏剑锋,杨锦春,董安涛..乌梅提取物对纳米细菌致大鼠肾结石形成的影响及机制探讨[J].山东医药,2017,57(14):14-17,4.基金项目
贵州省贵阳市科技计划项目(筑科合同20141001-45号). （筑科合同20141001-45号）
