山东医药2017，Vol.57Issue(14)：18-22,5.DOI:10.3969/j.issn.1002-266X.2017.14.005
围产期高脂高糖孕鼠NP暴露对仔鼠肥胖的影响及机制探讨
Effect of NP exposure on neonatal rat obesity of pregnant rats induced by high-fat and high-glucose diet during perinatal period
摘要
Abstract
Objective To observe the effect of nonylphenol (NP) exposure on offspring weight,fat generation and adipocyte differentiation genes,liver and adipocyte changes of pregnant rats induced by high-fat and high-sugar diet during perinatal period and to explore the mechanism of offspring obese occurrence via high-fat and high-glucose diet treatment combined with NP exposure in perinatal mothers.Methods Forty female SD rats were mated with 20 male SD rats.Then we collected the pregnant rats and divided them into the ordinary diet control group (OC),The high fat diet control group (HC),high fat+low-dose Nonylphenol group (HNL),and high fat + high-dose Nonylphenol group (HNH).Rats in the OC grup were fed with normal diet,and rats in the HC,HNL and HNH groups were fed with high fat and high sugar diet.Pregnant rats were given gestational gavage from day 6 to postpartum 21 days.In the OC group and HC group,the pregnant rats were given 0.5 mL/(kg·d) corn oil,in the HNL group and HNH,they were given NP 50,100 mg/(kg·d) by gavage.Pregnant rats were fed with normal breast feeding and fed with normal diet for up to 70 days after birth.After 70 days of intervention,the blood of the abdominal aorta of the offspring was taken and the levels of serum TG,TC,HDL and LDL were measured by automatic biochemical analyzer.Before taking blood,we measured the weight of offspring rats.After taking the blood,we took the gonadal fat pad to measure fat quality and calculate the fat coefficient.We collected the liver tissues of the offsprings,Using real-time PCR to detect the regulation of adipocyte differentiation key gene transcription factor (CEBP-α),sterol regulatory binding protein (SREBP-1),peroxisome proliferator-activated receptor γ (PPARγ) mRNA expression.Liver tissues and adipose tissues were observed by HE staining.Results The levels of serum TG,TC and LDL in the HNL group and HNH group were higher than those in the HC group (all P<0.01).The body mass and fat coefficient of the HNL group and HNH group were higher than those of the HC group,and the fat quality of the HNH group was higher than that of the HC group (all P<0.01).The expression of CEBP-α mRNA in the HNH group was higher than that in the OC group (P<0.01).The expression of SREBP-1 and PPARγ mRNA in the HNH group was higher than that in the HC group (all P<0.01).HE staining showed that some liver cells were steatosis in the HC group,and a large number of hepatocyte fat-like changes were seen in the HNL group.The extensive balloon-like and large amount of lipid droplets were found in the HNL group.In the HC group,part of the fat cell area increased,and in the HNL group,most of the fat cell area increased significantly.Conclusion High-fat and high-glucose diet combined with NP exposure on pregnant rats can increase the offspring body mass,fat quality and blood lipid levels,which lead to increasing the liver fat and adipocytes,and the mechanism may be that NP leads to the expression abnormalities of adipocyte differentiation genes CEBP-α,SREBP-1,and PPARγ.关键词
壬基酚/肥胖/围产期/脂肪Key words
nonylphenol/obesity/perinatal stage/lipid metabolism分类
医药卫生引用本文复制引用
李文梅,俞捷,罗娅,王攀,杨雪松,杨静,许洁..围产期高脂高糖孕鼠NP暴露对仔鼠肥胖的影响及机制探讨[J].山东医药,2017,57(14):18-22,5.基金项目
国家自然科学基金资助项目(81360439) （81360439）
贵州省教育厅青年基金(黔教合KY字[2013]198) （黔教合KY字[2013]198）
贵州省科技厅遵义医学院联合基金重点项目(黔科合LH[2014]7543) （黔科合LH[2014]7543）
遵义医学院2015年重点学科建设经费资助项目. （）
