山东医药2017，Vol.57Issue(14)：31-36,6.DOI:10.3969/j.issn.1002-266X.2017.14.008
不同浓度氧暴露对早产儿外周血单个核细胞SIRT1通路的影响
Effects of exposure to different concentrations of oxygen on SIRT1 pathway in peripheral blood mononuclear cells of premature infants
摘要
Abstract
Objective To observe the effects of exposure to different concentrations of oxygen at different time points on expression changes of SIRT1 pathway-related protein in peripheral blood mononuclear cells (PBMCs) of preterm infants with low body mass and to investigate the mechanism of oxidative stress injury induced by oxygen exposure.Methods Eighty cases of premature infants with low body mass,who were diagnosed with neonatal respiratory distress syndrome at admission and needed immediate oxygen therapy,were selected.According to the concentrations of oxygen inhalation,we divided them into the control group,low-concentration oxygen group,medium-concentration oxygen group,and high-concentration oxygen group,with 20 cases in each group.In the control group,the oxygen concentration (FiO2) was 21% or more than 21%,but the total oxygen time was less than 24 h.In the low-concentration oxygen group,FiO2 was more than 21%,and the total oxygen time was more than 24 h,or according to the patients′ condition the total oxygen time was less than 24 h with with FiO2>30%.In the medium-concentration oxygen group,FiO2 was from more than 30% to less than 40%,and the total oxygen time was more than 24 h,or according to the patients′ condition the total oxygen time was less than 24 h with FiO2>40%.In the high-concentration oxygen group,FiO2 was 40% or more than 40%,but the total oxygen time was more than 24 h.PBMCs were isolated from the peripheral blood samples (2 mL) collected from radial artery at day 0,7,14,and 28 after birth.SIRT1 localization was detected by immunofluorescence.The expression of SIRT1 protein,silent mating type information regulation 2 homolog-1 (SENP1) protein and acetylated p53 protein was detected in PBMCs by Western blotting.Results Compared with the control group,the reactive oxygen species (ROS) content in PBMCs increased gradually (P<0.05),the SIRT1 translocation rate increased gradually (P<0.05),the SIRT1 protein level in nucleus decreased significantly (P<0.05),the the SENP1 protein level in PBMCs increased (P<0.05),and the level of acetylated p53 protein in PBMCs increased (P<0.05) with the increase of oxygen concentration and prolonged hospital stay.Conclusion High concentrations of oxygen exposure and prolonged oxygen therapy can lead to large amounts of ROS produced by PBMCs in preterm infants with low body mass,and this mechanism may be related with the oxidative stress injury caused by the increased expression of SENP1 protein,the increased SIRT1 nuclear translocation and acetylation of p53 in PBMCs.关键词
吸氧/氧化应激/活性氧/沉默信息调节因子2相关酶1/SUMO特异性蛋白酶1/乙酰化p53蛋白/早产儿Key words
oxygen inhalation/oxidative stress/reactive oxygen species/silent mating type information regulation 2 homolog-1/SUMO-specific protease 1/acetylated p53 protein/premature infant分类
医药卫生引用本文复制引用
刘兴铃,董文斌,李清平,雷小平,张莲玉,卢佑英,赵帅..不同浓度氧暴露对早产儿外周血单个核细胞SIRT1通路的影响[J].山东医药,2017,57(14):31-36,6.基金项目
国家自然科学基金资助项目(81571480) （81571480）
中华儿科杂志第二届双鹤珂立苏科研基金资助项目(cjp2011-009) （cjp2011-009）
四川省教育厅科研基金资助项目(08ZA150) （08ZA150）
四川省卫生厅科研基金资助项目(90191). （90191）
