山东医药2017，Vol.57Issue(15)：1-4,4.DOI:10.3969/j.issn.1002-266X.2017.15.001
裂果薯总皂苷对人肝癌细胞增殖、迁移、凋亡的影响及对正常肝细胞的毒性作用
Effects of saponins from Schizocapsa plantaginea Hance on proliferation, migration and apoptosis of human hepatocellular carcinoma cells and its toxicity to normal hepatocytes
摘要
Abstract
Objective To investigate the effects of saponins from Schizocapsa plantaginea Hance (SFSP) on the proliferation, migration and apoptosis of human hepatocellular carcinoma SMMC-7721 cells and its toxicity to normal human hepatocytes HL-7702. Methods We chose the SMMC-7721 cells in the logarithmic phase and divided them into the administration group and the control group, cells in the administration group were treated with different concentrations of SFSP (0, 0.625, 1.25, 2.5 and 5 μg/mL) for 24 h. MTT assay was used to detect the proliferation inhibition rate of SMMC-7721 cells in order to finally calculate its half inhibitory concentration (IC50). SMMC-7721 cells in the logarithmic phase were treated with final concentrations of SFSP (0, 2, 4, and 6 μg/mL) for 24 h, and 48 h and then the scratch assay was used to observe the changes of cell migration ability. Furthermore, SMMC-7721 cells in the logarithmic phase were treated with final concentrations of SFSP (0, 2, 4, and 6 μg/mL) for 24 h, and meanwhile we observed their apoptosis and morphology by AO-EB double staining and immunofluorescence. We chose the HL-7702 cells in the logarithmic phase and separately treated them with different concentrations of SFSP (0, 2.5, 12.5, 25, 50 and 100 μg/mL) for 24 h. MTT assay was used to detect the proliferation inhibition rate of HL-7702 cells in order to finally calculate its IC50. Results After 0.625, 1.25, 2.5 and 5 μg/mL SFSP were applied to SMMC-7721 cells for 24, 48 and 72 h, the proliferation of cells was inhibited to different degrees. The inhibitory effect of SFSP on SMMC-7721 cells was in a time-and dose-dependent manner (all P<0.05). The IC50 values of SFSP at 24, 48 and 72 h were 3.75, 1.56 and 0.95 μg/mL, respectively. After 0, 2, 4, 6 μg/mL SFSP were applied to SMMC-7721 cells, the healing rate of the cells was gradually decreased and the apoptotic cells were gradually increased (all P<0.05). SMMC-7721 cells shrank, appeared round or spindle shape, tubulin β fluorescence intensity decreased and highly aggregated, and a large number of cells in the split cycle. After 0, 2.5, 12.5, 25, 50 and 100 μg/mL SFSP were applied to HL-7702 cells for 24 h, the proliferation of cells was inhibited to different degrees. The IC50 of SFSP was 47.6 μg/mL at 24 h, which was lower than that (3.75 μg/mL) of SMMC-7721 cells. Conclusions SFSP could significantly inhibit the proliferation and migration, induce the apoptosis, and block the cell cycle in human hepatocellular carcinoma SMMC-7721 cells. Meanwhile, SFSP have less toxic effect on normal human hepatocytes.关键词
裂果薯/总皂苷/肝癌/细胞增殖/细胞迁移/细胞凋亡Key words
Schizocapsa plantaginea Hance/total saponins/hepatocellular carcinoma/cell proliferation/cell migration/apoptosis分类
医药卫生引用本文复制引用
邱汉琛,梁钢,孙悦文,罗舜仁,陈燕燕,付丽香,廖智红,黄修影,刘布鸣,唐安洲..裂果薯总皂苷对人肝癌细胞增殖、迁移、凋亡的影响及对正常肝细胞的毒性作用[J].山东医药,2017,57(15):1-4,4.基金项目
国家自然科学基金资助项目(81460620) （81460620）
广西自然科学基金资助项目(2014GXNSFDA118025) （2014GXNSFDA118025）
广西中药质量标准研究重点实验室(广西壮族自治区中医药研究院)开放课题基金项目(桂中重开201105). （广西壮族自治区中医药研究院）
