山东医药2017，Vol.57Issue(15)：9-12,4.DOI:10.3969/j.issn.1002-266X.2017.15.003
miR-630在胃癌组织中的表达变化及对胃癌细胞SGC-7901增殖、侵袭能力的影响
Expression of miR-630 in gastric carcinoma and its effect on proliferation and invasion ability of gastric cancer cell line SGC-7901
摘要
Abstract
Objective To investigate the expression of miR-630 in gastric carcinoma and its effect on the proliferation and invasion ability of gastric cancer cell line SGC-7901 in vitro. Methods The gastric cancer tissues and adjacent normal gastric tissues were collected from 131 patients with gastric cancer. The expression of miR-630 in the tissues was detected by RT-PCR. The cultured SGC-7901 cells were divided into 3 groups: the experimental group, empty vector group and control group which were transfected with miR-630, empty vector and PBS, respectively. The cells were cultured for 12, 24, 48 and 72 h, and MTT assay was employed to test the proliferation ability of cells. At 24 h, Transwell assay was used to test the invasion ability of cells, and Western blotting was used to investigate the expression of p27, MMP-2, MMP-9 in SGC-7901 cells. Results The relative expression levels of miR-630 in the gastric cancer tissues and adjacent normal tissues were 1.93±0.41 and 2.74±0.48, respectively. The relative expression of miR-630 in the gastric cancer tissues was lower than that in the adjacent normal tissues (all P<0.05). The relative expression of miR-630 in gastric cancer patients with diameter ≥5 cm, with lymph node metastasis, in T2-T4 grade and in Ⅲ-Ⅳ phase was successively lower than that in patients with diameter >5 cm, without lymph node metastasis, in T2-T4 grade, and in Ⅲ-Ⅳ phase (all P<0.05). In vitro studies, at 24, 48 and 72 h after transfection, the cell proliferation ability of the experimental group was lower than that of the empty vector group and control group (all P<0.01). The transmembrane cells in the experimental group, empty vector group and control group were 15.4±2.7, 24.3±23.1 and 29.7±19.4, respectively. The number of the transmembrane cells in the experimental group was less than that in the empty vector group and the control group (all P<0.01). The relative expression of p27 protein in the the experimental group was higher, and the expression of MMP-2 and MMP-9 protein was lower than that in the empty vector group and control group (all P<0.01). Conclusions The expression of miR-630 in the gastric cancer tissues is decreased, and the low expression of mir-630 might be related to the development of gastric cancer. MiR-630 could inhibit the proliferation and invasion of gastric cancer cells, which may be related to the up-regulation of p27 and down-regulation of MMP-2 and MMP-9.关键词
胃癌/胃癌细胞/微小RNA/微小RNA630/细胞增殖/细胞侵袭Key words
gastric carcinoma/gastric carcinoma cells/miRNA/miR-630/cell proliferation/cell invasion分类
医药卫生引用本文复制引用
丁顺斌,王简勤,蔡明春,叶鑫,姜天华..miR-630在胃癌组织中的表达变化及对胃癌细胞SGC-7901增殖、侵袭能力的影响[J].山东医药,2017,57(15):9-12,4.基金项目
甘肃省卫生行业科研计划资助项目(GSWST2013-02). （GSWST2013-02）
