山东医药2017，Vol.57Issue(15)：17-20,4.DOI:10.3969/j.issn.1002-266X.2017.15.005
CYFIP1过表达的鼻咽癌细胞株CNE2增殖、迁移能力观察
Observation of proliferation and migration in nasopharyngeal carcinoma cells with over-expression of CYFIP1
摘要
Abstract
Objective To investigate the changes in the proliferation and migration of nasopharyngeal carcinoma cells with over-expression of cytoplasmic FMRP interacting protein 1 (CYFIP1). Methods A human nasopharyngeal carcinoma cell line (CNE2) was infected with a lentiviral vector containing CYFIP1 or the empty vector, which were used as the experimental group and blank group. The uninfected CNE2 cells acted as the control group. Cell proliferation was evaluated by MTT (represented by OD value) after cell culture for 24, 48, 72 and 96 h, separately. In clone formation assays, the cell culture was terminated when clones in plate could be seen by naked eyes and clones were counted. Cell migration was analyzed by Transwell chamber and Scratch assays. The cell migration distance and the number of penetrating cells were calculated. Results No significant difference was found in the OD value at different cell-culture time among these three groups (P>0.05). The number of clones in the experimental group, blank group and control group were 414±29, 426±30 and 381±59, respectively (P>0.05). The cell migration distance of the experimental group, blank group and control group was (6.71±0.1), (9.82±0.12) and (9.98±0.1) mm, respectively. Compared with the blank group and control group, the experimental group had a shorter migration distance (all P<0.05). The penetrating cells of the experimental group, blank group and control group were 37.33±1.53, 62.67±5.03 and 62.13±4.36. The experimental group had less penetrating cells than those of the blank group and control group (all P<0.05). Conclusions The cell migration of nasopharyngeal carcinoma cell line CNE2 with over-expression of CYFIP1 is inhibited. Abnormal expression of CYFIP1 maybe participates in the invasion and development of nasopharyngeal carcinoma.关键词
鼻咽癌/鼻咽癌细胞/胞质FMRP相互作用蛋白1/细胞增殖/细胞迁移Key words
nasopharyngeal carcinoma/nasopharyngeal carcinoma cells/cytoplasmic FMRP interacting protein 1/cell proliferation/cell migration分类
医药卫生引用本文复制引用
林中原,李音音,石祥,李文朝,莫丽军,黎小红,莫武宁..CYFIP1过表达的鼻咽癌细胞株CNE2增殖、迁移能力观察[J].山东医药,2017,57(15):17-20,4.基金项目
广西壮族自治区自然科学基金资助项目(2014GXNSFAA118228). （2014GXNSFAA118228）
