世界中医药2017，Vol.12Issue(4)：738-740,745,4.DOI:10.3969/j.issn.1673-7202.2017.04.006
C57BL/6J小鼠实验性自身免疫性脑脊髓炎模型建立的关键性问题
Key issues on establishment of the experimental autoimmune encephalomyelitis model in C57BL/6J mice
摘要
Abstract
Experimental autoimmune encephalomyelitis (EAE) model is an internationally recognized classic animal model of multiple sclerosis (MS) and is widely used in researches on the pathogenesis of MS and the evaluation of immunomodulator for MS.However, There are still many defects leading to morbidity less than 100% and high mortality.Therefore, in order to reach a high morbidity, a low mortality and a stable EAE model, the process of EAE model establishment was standardized and refined by improving the preparation, operation and nursing of the model immunization.In addition, the key issues to the successful establishment were analyzed and the solutions were provided according to the process of experiment operation.关键词
实验性免疫性脑脊髓炎模型/关键性问题/免疫前处理/免疫操作/免疫后护理Key words
Experimental autoimmune encephalomyelitis/Key issues/Preparation before immunization/Operation in immunization/Nursing after immunization分类
医药卫生引用本文复制引用
吴彦青,郭玉红,刘清泉..C57BL/6J小鼠实验性自身免疫性脑脊髓炎模型建立的关键性问题[J].世界中医药,2017,12(4):738-740,745,4.基金项目
国家自然科学基金项目(编号:81403221) （编号:81403221）
