世界中医药2017，Vol.12Issue(4)：861-864,869,5.DOI:10.3969/j.issn.1673-7202.2017.04.039
复方乌头微乳皮肤刺激性和急性毒性实验研究
Experimental Study on Acute Toxicity and Irritation of Compound Aconite Microemulsion Skin
摘要
Abstract
Objective:To observe the effect of compound aconite microemulsion skin irritation and acute toxicity test,to provide the basis for clinical drug safety.Methods:Single stimulation test selected 12 rats with intact skin and damaged skin of two groups,the same side of 24 h,before the experiment will be on both sides of the back symmetrical with depilatory.The hair part is divided into 4 parts,the left side is divided into 2 regions,the right side is divided into upper and lower regions,in the rat body left on the skin with micro 0.5 mL,right side of the skin with the rest of the blank microemulsion 0.5 mL,without treatment,to remove drug applicator for 6 hours,respectively 1、24、48、72 h observation of skin irritation reaction and recovery,compared to the contralateral side;multiple stimulation experiment method ibid,administered 3 times daily,0.5 mL each time,for 7 d,at the end of the administration after drug removal within a week,view Observation of skin irritation;acute toxicity test:SD rats were randomly divided into 5 groups:normal saline group,blank microemulsion group,high,medium and low dose group,15 rats in each group,half male and half female.The rats back on both sides of the spine to shave,an area of about 4 cm×3 cm,the blank control group with saline 0.9 mL/(d only),the drug control group with Vaseline ointment(only 0.9 mL/D),low dose group with micro 0.9 mL/(only D),middle dose group(1.5 mL/TU microemulsion-only D),high dose group with 2.5 mL/microemulsion(only D),divided into 3 doses.The interval of 4 hours,continuous administration of 14 days(7 days apart,a hair removal)before administration(D0),seventh days after administration(D7),after administration of l the 4 day(D14)records of animal weight,observe the appearance of animal signs,behavior,glandular secretion,and detection of blood and blood biochemical index.Results:Skin irritation test showed no obvious change index,biochemical index compared with the control group there were no significant differences in skin acute toxicity test of majorhematologic(P>0.05)high there are two dose group diarrhea rats,weight loss,appearance,the rest rats behavioral signs,activities and mental status were not significantly abnormal;diet,feces and urine of normal rats;no occurrence of death.Conclusion:Compound aconite microemulsion had no irritation to the rats with intact skin,mild irritation to skin damage,no acute toxicity on the skin of rats.关键词
复方乌头微乳/动物/实验/透皮给药/安全性Key words
Compound aconite microemulsion/Animal/Experiment/Transdermal drug delivery/Safety分类
医药卫生引用本文复制引用
李玉奎,赵爱侠,赵琳,马振勇..复方乌头微乳皮肤刺激性和急性毒性实验研究[J].世界中医药,2017,12(4):861-864,869,5.基金项目
河南省教育厅科研课题(编号:15B360009) （编号:15B360009）
