兽类学报2016，Vol.36Issue(2)：221-231,11.DOI:10.16829/j.slxb.201602011
高原鼢鼠prestin基因的组织特异性表达与适应地下生活的关系
The relationship between the structure of the prestin gene and specific expression in tissues of the plateau zokor and its adaption to the underground life
摘要
Abstract
The Plateau zokor (Eospalax baileyi) is a typical subterranean mole rat in Qinghai-Tibet plateau.Subterranean mole rat is a kind of animal which has the ability to orient space accurately.Prestin expressed specifically in cochlea is the protein related to the echolocation.In order to shed light on the relationship between prestin and underground spatial orientation of plateau zokor,the CDS sequence of plateau zokor prestin gene was cloned and compared with other species.The software PAML was used to analyze the adaptive evolution of amino acid sites in the prestin of plateau zokor.The expression levels of prestin mRNA in cochlea,tail,paw and nose were determined with real-time PCR according to the cloned sequence.The results indicated that the CDS sequence of the plateau zokor prestin was similar to those of six kinds of mammals such as human,rat,mouse,naked mole rat,rabbit and cow;and the amino acid sequences showed a relatively high degree of conservation with nine particular amino acid substitutions.No significantly positive selection site was detected with Test 2 model.The expression level ofprestin mRNA in plateau zokor cochlea was significantly higher than that in plateau pika (P ＜ 0.05),and the prestin mRNA in plateau zokor cochlea was significantly higher than that in the paw and nose (P ＜ 0.01).The resuh above suggested that prestin not only is expressed in the cochlea,but also in paw,nose and tail of plateau zokor.Plateau zokors may perceive low frequency sound waves to accurately orient the space with assistance from their paws,noses and tails during their underground trunk life.关键词
高原鼢鼠/Prestin基因/组织特异性表达/地下空间定位Key words
Plateau zokor (Eospalax baileyi)/Prestin gene/Tissue specific expression/Subterranean spatial orientation分类
生物科学引用本文复制引用
许利娜,魏琳娜,汪洋,李筱,魏莲,魏登邦..高原鼢鼠prestin基因的组织特异性表达与适应地下生活的关系[J].兽类学报,2016,36(2):221-231,11.基金项目
国家自然科学基金项目(31260512) （31260512）
青海省自然科学基金项目(2012-Z-905及2014-ZJ-714) （2012-Z-905及2014-ZJ-714）
