兽类学报2017,Vol.37Issue(2):200-206,7.DOI:10.16829/j.slxb.201702011
利用微卫星研究普洱亚洲象种群的遗传多样性
Genetic diversity analysis based microsatellite of Asian elephant in Puer
摘要
Abstract
Using a non-invasive method,113 fecal samples from the Asian elephant population in Puer were collected.After getting total DNA using kits,specific amplification by 9 microsatellite loci resulted in a 49 unique genotypes which were identified by CERVUS 3.0.The analysis of genetic diversity through GenAlEx v6.5 and Arlequin v3.5 showed that loci did not deviate from Hardy-Weinberg equilibrium;the average allele number was 3.111;the average Shannon's Information Index was 0.804;the average expected heterozygosity was 0.468 and the average observed heterogosity was 0.476.Comparisons of genetic heterozygosity from different areas showed that the genetic diversity of Asian elephant in Puer was low.关键词
普洱/亚洲象/微卫星/遗传多样性Key words
Asian elephant/Genetic diversity/Microsatellite/Puer分类
生物科学引用本文复制引用
杜娇娇,何长欢,朱頔,杨帆,张立..利用微卫星研究普洱亚洲象种群的遗传多样性[J].兽类学报,2017,37(2):200-206,7.基金项目
国家自然科学基金资助项目(31172108) (31172108)