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利用微卫星研究普洱亚洲象种群的遗传多样性

杜娇娇 何长欢 朱頔 杨帆 张立

兽类学报2017,Vol.37Issue(2):200-206,7.
兽类学报2017,Vol.37Issue(2):200-206,7.DOI:10.16829/j.slxb.201702011

利用微卫星研究普洱亚洲象种群的遗传多样性

Genetic diversity analysis based microsatellite of Asian elephant in Puer

杜娇娇 1何长欢 1朱頔 1杨帆 1张立1

作者信息

  • 1. 北京师范大学生命科学学院,教育部生物多样性与生态工程重点实验室,北京100875
  • 折叠

摘要

Abstract

Using a non-invasive method,113 fecal samples from the Asian elephant population in Puer were collected.After getting total DNA using kits,specific amplification by 9 microsatellite loci resulted in a 49 unique genotypes which were identified by CERVUS 3.0.The analysis of genetic diversity through GenAlEx v6.5 and Arlequin v3.5 showed that loci did not deviate from Hardy-Weinberg equilibrium;the average allele number was 3.111;the average Shannon's Information Index was 0.804;the average expected heterozygosity was 0.468 and the average observed heterogosity was 0.476.Comparisons of genetic heterozygosity from different areas showed that the genetic diversity of Asian elephant in Puer was low.

关键词

普洱/亚洲象/微卫星/遗传多样性

Key words

Asian elephant/Genetic diversity/Microsatellite/Puer

分类

生物科学

引用本文复制引用

杜娇娇,何长欢,朱頔,杨帆,张立..利用微卫星研究普洱亚洲象种群的遗传多样性[J].兽类学报,2017,37(2):200-206,7.

基金项目

国家自然科学基金资助项目(31172108) (31172108)

兽类学报

OA北大核心CSCDCSTPCD

1000-1050

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