兽类学报2017，Vol.37Issue(2)：207-214,8.DOI:10.16829/j.slxb.201702012
牦牛SND1基因特征序列分析及其蛋白在乳腺的表达
The characteristics of yak SND1 gene and its expression in mammary gland
摘要
Abstract
Staphylococcal nuclease and tudor domain containing 1 (SND1,Tudor-SN),a transcriptional co-activator protein,is involved in the regulation of gene expression.The objective of this study was to analyze the biological characteristic of yak SND1,and to examine the expression of SND1 in mammary gland.Mammary samples from local yak were collected and primary mammary epithelial cells were obtained by trypsin digestion.The third generation of purified mammary epithelial cells was used to clone yak SND1 gene by RT-PCR.Bio-information software was used to analyze the characteristics of SND1,and immunohistochemistry and immunofluorescence were used to detect the location of protein encoded by SND1.The results showed that the coding region of Bos grunniens SND1 gene was 3294 bp in length,encoding 20 amino acids.The encoded protein of SND1 gene was predicted to be a type of non-secreted and non-transmembrane protein.The homology of SND1 gene between yak and Bison,Bos taurus,Pantholops hodgsonii,Capra hircus,Sus scrofa,Camelus ferus,Equus caballus,Pan paniscus,Homo sapiens,Rattus norvegicus were 99％,98％,96％,94％,91％,90％,90％,89％,89％,85％,respectively.It was found that,in evolution,the SND1 gene of yak was closely related to those of Bison and Bos taurus,and distantly related to that of Homo sapiens and Rattus norvegicus.In yak mammary gland,the SND1 had high positive expression in the secretory epithelial cells (mammary epithelial cell) and ductal epithelial cells,but weak expressionin myo-epithelial cells.SND1 protein was highly expressed in the nuclei of mammary epithelial cells and weakly expressed in cytoplasm.In summary,our study could provide a theoretical basis for further study of yak lactation function,and also provide reference data for the mammals living in Qinghai-Tibet Plateau.关键词
牦牛/SND1/乳腺上皮细胞/生物学特性/表达Key words
Biological characteristic/Expression/Mammary epithelial cells/SND1/Yak分类
生物科学引用本文复制引用
田奎丽,潘阳阳,李月琴,崔燕,余四九..牦牛SND1基因特征序列分析及其蛋白在乳腺的表达[J].兽类学报,2017,37(2):207-214,8.基金项目
国家自然科学基金(31472244) （31472244）
甘肃省生物技术专项(GNSW-2013-23) （GNSW-2013-23）
