水生生物学报2017，Vol.41Issue(3)：506-515,10.DOI:10.7541/2017.65
玉米蛋白粉替代鱼粉对大黄鱼生长、血清生化指标及肝脏组织学的影响
EFFECTS OF REPLACING FISH MEAL WITH CORN GLUTEN MEAL ON THE GROWTH, SERUM BIOCHEMICAL INDICES AND LIVER HISTOLOGY OF LARGE YELLOW CROAKERLARIMICHTHYS CROCEA
摘要
Abstract
To investigate the effects of replacing fish meal by corn gluten meal (CGM) on fish growth, serum bioche-mical indices and liver histology, six isonitrogenous (45% crude protein) and isolipidic (10% crude lipid) diets with various dietary protein levels (0, 15%, 30%, 45%, 60% and 75%) (designated as C0, C15, C30, C45, C60, C75) were formulated to feed triplicate groups of juvenile large yellow croaker [mean initial weight: (10.49±0.03) g] for a 56 days trail. In addition, all diets except the control diet were supplemented with crystalline amino acids such as lysine and me-thionine. Results showed that the survival rate (SR), specific growth rate (SGR) and feed conversion ratio (FCR) were not significantly affected (P>0.05). Muscle crude protein content in C45 and C60 were significantly higher than that of C0 (P<0.05). Muscle crude lipid content in C45 was significantly higher than that of C0, C15 and C75 (P<0.05). Muscle moisture in C45, C60 and C75 were significantly lower than that of C0. CGM had no effects on crude protein, crude lipid and moisture of whole body (P>0.05), while, the increased CGM replacement enhanced the whole body ash. CGM did not impact serum ALB, A/G, AST and GLU. The increasing dietary CGM level decreased total TC with a significant difference when the substitution level was more than 30% (P<0.05). The serum TP and GLB content of C0 was significantly higher than that of C75 (P<0.05). Serum AST of C60 and C75 were significantly higher than that of C0 (P<0.05) with the highest in the C75. No significant differences were observed in the T-AOC, CAT and GHS-Px by CGM (P>0.05). While CGM can increase the T-AOC content. Compared to C0 diet, C75 had significant and highest value in MDA (P<0.05). From the liver histology, replacement proportion of fish meal by CGM more than 45% media-ted liver cell nucleus location and increased lipids accumulation that were translucent with vacuole. These results sug-gest that the optimal dietary protein level in juvenile large yellow croaker is about 45%.关键词
大黄鱼/玉米蛋白粉/生长性能/血清生化指标/抗氧化/肝脏组织学Key words
Large yellow croaker/Corn gluten meal/Growth performance/Serum biochemical indices/Antioxidase/Liver histology分类
农业科技引用本文复制引用
何娇娇,王萍,冯建,娄宇栋,党慧,邓蓉..玉米蛋白粉替代鱼粉对大黄鱼生长、血清生化指标及肝脏组织学的影响[J].水生生物学报,2017,41(3):506-515,10.基金项目
浙江省自然科学重点基金(Z16E090006) （Z16E090006）
国家海洋公益性行业科研专项(201505025) （201505025）
舟山市海洋专项(2015C41001) （2015C41001）
浙江省重中之重学科开放基金(XKZSC1406) （XKZSC1406）
国家自然科学基金(31602205) （31602205）
浙江海洋大学省重中之重学科项目(20161105)资助Supported by the National Natural Science Foundation of Zhejiang Province (Z16E090006) （20161105）
Public Science and Technology Research Funds Projects of Ocean (201505025) （201505025）
the Special Funds Projects of Ocean in Zhoushan, Zhejiang Province (2015C41001) （2015C41001）
the Key Discipline Open Fund of Zhejiang Province (XKZSC1406) （XKZSC1406）
the National Science Foundation of China (31602205) （31602205）
the Key Discipline Projects for Zhejiang Ocean University of Zhejiang Province (20161105) （20161105）
