水生生物学报2017，Vol.41Issue(3)：516-522,7.DOI:10.7541/2017.66
氨氮对中华小长臂虾的急性毒性及非特异性免疫指标的影响
EFFECT OF AMMONIA NITROGEN ON ACUTE TOXICITY AND NON-SPECIFIC IMMUNE PARAMETERS OFPALAEMONETES SINENSIS
摘要
Abstract
The current study explored the acute biological toxicity of ammonia-N onPalaemonetes sinensis. In the con-dition of (18±1)℃, pH 7.3±0.1, the half lethal concentrations of ammonia-N were 565.47 mg/L for 24h, 371.16 mg/L for 48h, 291.16 mg/L for 72h, and 272.50 mg/L for 96h, respectively, and the safe concentration was 27.25 mg/L. In the same condition, half lethal concentrations of non-ionic ammonia were 3.74 mg/L for 24h, 2.45 mg/L for 48h, 1.93 mg/L for 72h, 1.80 mg/L for 96h respectively and the safe concentration was 0.18 mg/L. Five different concentrations (60 mg/L, 100 mg/L, 140 mg/L, 180 mg/L and 220 mg/L) were used to study the influence of ammonia nitrogen stress on the non-specific immune indexes based on 96hLC50 and safety concentration. Results showed that the superoxide dismutase (SOD) in hepatopancreas and muscle of P. sinensis significantly increased by ammonia nitrogen at 24h ex-cept muscle tissue in 220 mg/L, which decreased to the normal level at 48—96h. Compared with the control group, acid phosphatase (ACP) in hepatopancreas of P. sinensis by ammonia nitrogen treatment was not changed significantly at 24h, while alkaline phosphatase (AKP) was significantly increased. At 48—96h, both ACP and AKP were lower than the control group. Muscle ACP and AKP by ammonia nitrogen treatments except 140 mg/L were significantly lower than those in the control group at 24 and 96h. This study revealed thatP. sinensis had strong tolerance to ammonia ni-trogen. High ammonia nitrogen had can inhibit the activities of immune enzyme. The results will provide the scientific basis for the aquaculture of P. sinensis.关键词
中华小长臂虾/氨氮/急性毒性/SOD/ACP/AKPKey words
Palaemonetes sinensis/Ammonia-N/Acute toxicity/SOD/ACP/AKP分类
农业科技引用本文复制引用
包杰,姜宏波,程慧,于业辉,李晓东..氨氮对中华小长臂虾的急性毒性及非特异性免疫指标的影响[J].水生生物学报,2017,41(3):516-522,7.基金项目
辽宁省教育厅项目(L2015472、L2014254) （L2015472、L2014254）
辽宁省自然科学基金(201602654)资助 Supported by the fund of Liaoning Provincial Department of Education (L2015472, L2014254) （201602654）
Natural Foundation of Liaoning Province (201602654) （201602654）
