水生生物学报2017，Vol.41Issue(3)：581-588,8.DOI:10.7541/2017.75
基于线粒体COⅠ基因和控制区序列的辽宁沿海弯棘斜棘(鱼衔)群体遗传多样性和遗传结构分析
POPULATION GENETIC STRUCTURE AND DIVERSITY ANALYSIS OF REPOMUCENUS CURVICORNIS IN THE LIAONING COAST BASED ON DNA SEQUENCES OF THE MITOCHONDRIALCOⅠ GENE AND CONTROL REGION
摘要
Abstract
To analyze the genetic diversity and genetic structure ofRepomucenus curvicornis from the Liaodong Bay (n=22) and the North Yellow Sea (n=18), the mitochondrial DNA cytochrome oxidase I (COⅠ) gene and control re-gion fragments were obtained by PCR amplification. The average contents of A, T, C and G of 624 bpCOⅠgene frag-ments were 24.09%, 31.04%, 25.28%, and 19.59%, respectively. The average contents of A, T, C and G of CR frag-ments (460 bp) were 32.96%, 32.80%, 14.86% and 19.38%, respectively. The total variable sites, mean pairwise nucle-otide differences (k), haplotype diversity (H) and nucleotide diversity (π) based onCOⅠgene fragments were 38, 4.67, 0.96±0.02 and 0.0075±0.0042, respectively. The same parameters based on CR fragments were 26, 3.35, 0.97±0.02 and 0.0073±0.0043, respectively. Based on mitochondrialCOⅠgene and control region, the genetic diversity of Liaodong Bay population was lower than that of North Yellow Sea population. The AMOVA analysis based on CR fragments re-vealed almost significant genetic divergence between the Liaodong Bay and North Yellow Sea populations, while there was no significant genetic divergence based onCOⅠgene. The results showed that CR andCOⅠgene were effective molecular markers for detecting the genetic diversity ofRepomucenus curvicornis population, while CR was more relia-ble thanCOⅠgene in detecting the genetic structure. In conclusion, CR is a appropriate marker for marine fish popula-tion genetic analysis.关键词
弯棘斜棘(鱼衔)/线粒体DNA/COⅠ基因/控制区序列/遗传多样性/遗传分化Key words
Repomucenus curvicornis/mtDNA/COⅠgene/Control region/Genetic diversity/Genetic differentiation分类
生物科学引用本文复制引用
李玉龙,刘修泽,于旭光,李轶平,付杰,董婧..基于线粒体COⅠ基因和控制区序列的辽宁沿海弯棘斜棘(鱼衔)群体遗传多样性和遗传结构分析[J].水生生物学报,2017,41(3):581-588,8.基金项目
海洋公益性行业科研专项(201405010) （201405010）
辽宁省海洋与渔业科研项目(201401)资助 Supported by the Public Science and Technology Research Funds Project of Ocean (201405010) （201401）
the Research Project of Marine and Fishery of Liaoning Province (201401) （201401）
