生态学报2017，Vol.37Issue(6)：1749-1756,8.DOI:10.5846/stxb201511122292
土壤病毒生态学研究方法
Research methods for soil viral ecology
摘要
Abstract
Viruses are the most abundant biological entities on the planet,and can reach 1010 viral particles per gram of soil.Viruses affect the composition of microbial communities,mediate soil biogeochemical cycles,regulate soil microbial evolution,and threaten the health of plants and animals,including humans.However,there is limited knowledge on the abundance and distribution patterns of viruses and the manner in which they affect ecological processes.This study focuses on the comparative approaches to study soil viruses,including their extraction,purification,quantification,and methods pertaining to molecular ecology.The development of reliable and highly efficient methods to evaluate soil viral particles is desirable,because it aids the study of soil virus diversity and distribution.Therefore,these methods will improve our understanding of viral propagation mechanisms in soils,and will provide information on how to control viruses to ensure adequate public health and safety.关键词
土壤病毒/病毒生态学/宏病毒组学/分子生物学方法Key words
soil virus/viral ecology/metaviromics/molecular biological methods引用本文复制引用
韩丽丽,于丹婷,贺纪正..土壤病毒生态学研究方法[J].生态学报,2017,37(6):1749-1756,8.基金项目
国家自然科学基金资助项目(41571248,41301265,41230857) （41571248,41301265,41230857）
