生态学杂志2017，Vol.36Issue(4)：978-987,10.DOI:10.13292/j.1000-4890.201704.003
三江源区不同建植年限对人工草地土壤微生物功能多样性的影响
Effects of cultivation duration on soil microbial functional diversity of artificial grassland in the Three-River Headwater Region
摘要
Abstract
The objective of the current study is to reveal the changes of soil microbial functional diversity in the artificial grassland with different cultivation periods in the Three-River Headwater Region,so as to provide evidence to ameliorate the degraded alpine meadow based on the efforts of planting artificial grassland.Three artificial grasslands with cultivation durations of 4,8 and 12 years were selected.Soil nutrients and microbial functional diversity were determined using conventional laboratory analysis and the Biolog-ECO microplate method.In the 0-10 cm soil layer,soil pH and total nitrogen (TN) content were significantly greater with 4 cultivation years than with the other cultivation years,while total potassium (TK) and available nutrients increased with increasing cultivation years (P＜0.05).In the 10-20 cm soil layer,soil pH was significantly higher in 4-year grassland than in the other grasslands,while TK in 12-year grassland was significantly lower than in the other grasslands (P＜0.05).Additionally,available nitrogen (AN) and available potassium (AK) showed the following pattern:8 years ＞ 12 years ＞ 4 years.In the 0-10 cm soil layer,average well color development (AWCD) value showed 8 years ＞ 12 years ＞ 4 years.But AWCD value increased with increasing cultivation years in the 10-20 cm soil layer.Soil microbial functional diversity showed that Shannon and Pielou index in 0-10 cm soil layer of the 12-year grassland were lowest,while in the 10-20 cm soil layer the least values were found in the 4-year grassland.McIntosh index increased with increasing cultivation years in the 10-20 cm soil layer.Principal component analysis (PCA) showed that amino acids were the main carbon source for maintaining soil microbial community.Redundancy analysis (RDA) indicated that soil organic carbon (SOC),TK,AN,AK and pH were the main factors influencing the metabolic rate of soil microbial community and microbial functional diversity.In summary,there were differences among soil microbial functional diversity in the artificial grasslands of different cultivation durations.Soil nutrient content,soil microbial community stability and ecological environment improved with increasing cultivation years.关键词
人工草地/土壤微生物群落/建植年限/Biolog-ECOKey words
artificial grassland/soil microbial community/cultivation duration/Biolog-ECO引用本文复制引用
字洪标,刘敏,阿的鲁骥,胡雷,王长庭..三江源区不同建植年限对人工草地土壤微生物功能多样性的影响[J].生态学杂志,2017,36(4):978-987,10.基金项目
国家自然科学基金项目(31370542)、国家重大科学研究计划项目(2013CBA01807)和中央高校基本科研业务费优秀科研及重大孵化项目(2014NZYTD01)资助. （31370542）
