生物骨科材料与临床研究2017，Vol.14Issue(2)：1-4,4.DOI:10.3969/j.issn.1672-5972.2017.02.001
BMP-14诱导骨髓间充质干细胞向肌腱细胞分化的实验研究
Experimental study on bone marrow mesenchymal stem cells induced to tendon cells by BMP-14
黄晓楠1
作者信息
- 1. 山东菏泽市立医院手足显微外科,山东菏泽274031
- 折叠
摘要
Abstract
Objective To explore bone marrow mesenchymal stem cells (BMSCs) induced to tendon cells by BMP-14,provided methods of obtaining tendon tissue engineering seed cells.Methods The BMSCs obtained from adult rabbit was cultured in the establishment of two group-experimental group (using BMP-14 induced culture) and control group (using natural differentiation).Use collagen type Ⅰ immunohistochemical and toluidine blue staining.Detect the expression of mRNA of proteoglycan and collagen type Ⅰ protein by RT-PCR at different time during culture.Results Immunohistochemistry staining ofproteoglycan and collagen type Ⅰ protein in BMSCs climbing film showed positive,while the control group showed negative.RT-PCR results suggest that the induced BMSCs express proteoglycan and collagen type Ⅰ protein higher than the control group.Conclusion BMP-14 can induce the BMSCs differentiating into the tendon cells in vitro.关键词
骨髓间充质干细胞/肌腱细胞/BMP-14Key words
BMSCs/Tendon cells/BMP-14分类
医药卫生引用本文复制引用
黄晓楠..BMP-14诱导骨髓间充质干细胞向肌腱细胞分化的实验研究[J].生物骨科材料与临床研究,2017,14(2):1-4,4.
