SMYD2在反复自然流产患者子宫内膜蜕膜化中的作用及机制OACSTPCD
Role of SMYD2 in endometrial decidualization of recurrent spontaneous abortion patients and its mechanism
目的 探讨SMYD2在反复自然流产(RSA)患者子宫内膜基质细胞和蜕膜化细胞中的表达及其对P53、LIF的调节作用. 方法 将正常人和RSA患者子宫内膜基质细胞进行体外诱导蜕膜化处理,并分为空白组、蜕膜化组、蜕膜化+抑制剂组,采用real-time PCR检测LIF、SMYD2、TRP53 mRNA,细胞免疫化学法检测SMYD2、P53、P-P53蛋白在子宫内膜蜕膜化细胞的表达及SMYD2特异性抑制剂AZ505对其表达的影响. 结果 在正常人和R…查看全部>>
Objective To investigate the expression of SMYD2 in stromal cells and decidual cells of patients with recurrent spontaneous abortion(RSA),and its regulation effect on P53 and LIF expression.Methods The endometrial stromal cells of normal and RSA patients was decidualized in vitro,and the cells were divided into blank control group,decidualization group and decidualization+inhibitor group.Real-time PCR and immunocytochemistry were used to observe the mRNA and…查看全部>>
杨慧;张昌军;刁红录
武汉大学中南医院妇产科,武汉 430071湖北医药学院附属人民医院生殖医学中心武汉大学中南医院妇产科,武汉 430071
医药卫生
SMYD2P53LIF反复自然流产子宫内膜蜕膜化
SMYD2P53LIFrecurrent spontaneous abortionendometriumdecidualization
《山西医科大学学报》 2017 (4)
337-342,6
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