实用医学杂志2017，Vol.33Issue(3)：358-362,5.DOI:10.3969/j.issn.1006-5725.2017.03.007
糖基化终产物通过其受体激活NADPH氧化酶及其下游通路诱导L细胞的凋亡
Advanced glycation end products and its receptor induce apoptosis of L cells through NADPH oxidase mediated signaling pathway
摘要
Abstract
Objective To investigate the effects of AGEs-RAGE on the apoptosis of GLUTag cells and explore the possiblc mechanism.Methods GLUTag cells treated with 0、100、200、300μg/ml of AGEs for 24h were examined for gene and protein expression of RAGE using RT-PCR and western blotting,respectively.GLUTag cells were randomly divided into four groups:control,200μg/ml AGEs,AGEs+siRNA-RAGE and AGEs+apocynin.The protein expression of p22phox、p47phox 、Bcl-2、Bax in the cells were detected with western blotting.The reactive oxygen species (ROS) levels were examined using 2'7'-dichlorodihydroflur-rescein diacetate (DCFH-DA) and the apoptosis of L cells were tested by AnnexinV-FITC/PI.Results AGEs increased thc cxpression of RAGE in a dose dependent manner.Treatment with AGEs induced a significant increase in the expression of p22phox,p47phoxand the activity of ROS,caused up-regulation of Bax and down-regulation of Bcl-2,which enhanced the apoptosis of GLUTag cells.Apocynin,the inhibitor of NADPH oxidase,prevented those responses and the effects caused by AGEs were abolished by inhibition of RAGE activity with siRNA.Conclusion AGEs positively regulate the exprcssion of NADPH oxidase-derived ROS and its down-steam signaling pathway p53/Bax by targeting RAGE,leading to the apoptosis of GLUTag cells.关键词
糖基化终产物/肠道L细胞/凋亡/NADPH氧化酶/活性氧Key words
Advanced glycation end-products/GLUTag cells/Apoptosis/NADPH oxidase/Reactive oxidative species引用本文复制引用
胡颖辉,张振,雷蕾,杨锐,林江虹,曾惠娴,陈宏..糖基化终产物通过其受体激活NADPH氧化酶及其下游通路诱导L细胞的凋亡[J].实用医学杂志,2017,33(3):358-362,5.基金项目
国家自然科学基金青年基金项目(编号:81500623) （编号:81500623）
广东省科技计划项目(编号:2014A020212489) （编号:2014A020212489）
