实用医学杂志2017，Vol.33Issue(5)：680-683,4.DOI:10.3969/j.issn.1006⁃5725.2017.05.002
锰对SH⁃SY5Y细胞线粒体超微结构及功能改变的研究
Mitochondrial ultrastructure and function changes in SH?SY5Y induced by manganese
摘要
Abstract
Objective To explore the mechanism of SH?SY5Y mitochondrial dysfunction treated by manganese to find a new potential therapeutic target. Methods Transmission Electron Microscopy(TEM)to observe the morphology of mitochondria. Cell treated with 250μmol/L for periods of time(2 h, 4 h, 6 h)while mitochondrial membrane potential(MMP)and ROS can be detected by FCM and fluorescence microplate reader. Results After treating with MnCl2 in 6 h, TEM images showed early vacuoles, lamellar structures of SH?SY5Y cells. Then test the mitochondrial membrane potential and showed that MMP would be decreased gradually. Meanwhile, analysis showed that in comparison with control, treatment group had a higher ROS level respectively (P < 0.05). Conclusion MnCl2 can cause mitochondrial damage through a mechanism closely related to disrupt the MMP or generate abundant ROS.关键词
锰/线粒体/超微结构/线粒体膜电位Key words
Manganese/SH⁃SY5Y cell line/Mitochondria dysfunction引用本文复制引用
宋冬梅,陈丽,马俊香,张媛媛,张诗玄,郭彩霞,陈田,田琳,牛丕业..锰对SH⁃SY5Y细胞线粒体超微结构及功能改变的研究[J].实用医学杂志,2017,33(5):680-683,4.基金项目
国家自然科学基金项目(编号:81673137) （编号:81673137）
北京市自然科学基金项目(编号:7172024) （编号:7172024）
北京市教育委员会高等学校高层次人才引进与培养及创新团队建设计划项目(编号:CIT&TCD201504094) （编号:CIT&TCD201504094）
